Detailed information of TA system TA212861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsaAT/-
• Location: 887870..888347
• Replicon: chromosome
• Accession: NZ_CP081354
• Organism: Staphylococcus aureus strain SQL1

Toxin (Protein)

• Gene name: TsaT
• Uniprot ID: Q2G005
• Locus tag: K1X69_RS04255
• Protein ID: WP_000052078.1
• Coordinates: 887870..888058 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: TsaA
• Uniprot ID: T1Y6P8
• Locus tag: K1X69_RS04260
• Protein ID: WP_001059690.1
• Coordinates: 888087..888347 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K1X69_RS04225 (883911)	883911..884381	+	471	WP_000824472.1	hypothetical protein	-
K1X69_RS04230 (884722)	884722..885408	+	687	WP_001548082.1	thermonuclease family protein	-
K1X69_RS04235 (885765)	885765..885965	+	201	WP_001059082.1	cold-shock protein	-
K1X69_RS04240 (886461)	886461..886679	-	219	WP_000005886.1	hypothetical protein	-
K1X69_RS04245 (886741)	886741..887025	-	285	WP_001004395.1	hypothetical protein	-
K1X69_RS04250 (887113)	887113..887682	-	570	WP_001106654.1	lipoprotein N-acylation protein LnsA	-
K1X69_RS04255 (887870)	887870..888058	-	189	WP_000052078.1	hypothetical protein	Toxin
K1X69_RS04260 (888087)	888087..888347	-	261	WP_001059690.1	hypothetical protein	Antitoxin
K1X69_RS04265 (888688)	888688..888891	+	204	WP_000145163.1	sterile alpha motif-like domain-containing protein	-
K1X69_RS04270 (888888)	888888..889124	-	237	WP_000368687.1	hypothetical protein	-
K1X69_RS04275 (889349)	889349..889930	+	582	WP_001140686.1	histidine phosphatase family protein	-
K1X69_RS04280 (890003)	890003..890620	-	618	WP_000255561.1	LysE/ArgO family amino acid transporter	-
K1X69_RS04285 (890775)	890775..891269	-	495	WP_000569884.1	GNAT family N-acetyltransferase	-
K1X69_RS04290 (891668)	891668..892090	-	423	WP_000974460.1	organic hydroperoxide resistance protein	-
K1X69_RS04295 (892238)	892238..892954	+	717	WP_000150017.1	type I 3-dehydroquinate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	clpP / vWbp / selk / selq	838768..913636	74868

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7172.84 Da
• Isoelectric Point: 9.9971

>T212861 WP_000052078.1 NZ_CP081354:c888058-887870 [Staphylococcus aureus]
MSLHFAILFWLALIFLVAATFILVLMKKTSKESKKESYLSFTVILYIFGFAILIYTFIFGVL

Nucleotide

• Length: 189 bp

>T212861 NZ_CP107383:4183428-4183646 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9940.09 Da
• Isoelectric Point: 4.0624

>AT212861 WP_001059690.1 NZ_CP081354:c888347-888087 [Staphylococcus aureus]
MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICGVILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPI
FYSFIG

Nucleotide

• Length: 261 bp

>AT212861 NZ_CP107383:4183027-4183401 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0D6HZ40

Antitoxin

• AlphaFold DB: A0A0E0VN56