Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4545870..4546489 Replicon chromosome
Accession NZ_CP081351
Organism Klebsiella michiganensis strain CCRI-24235

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag Km24235_RS21375 Protein ID WP_004099646.1
Coordinates 4546271..4546489 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID H3N7X7
Locus tag Km24235_RS21370 Protein ID WP_004129911.1
Coordinates 4545870..4546244 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Km24235_RS21360 (Km24235_4237) 4541027..4542220 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
Km24235_RS21365 (Km24235_4238) 4542243..4545389 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit AcrB -
Km24235_RS21370 (Km24235_4239) 4545870..4546244 + 375 WP_004129911.1 Hha toxicity modulator TomB Antitoxin
Km24235_RS21375 (Km24235_4240) 4546271..4546489 + 219 WP_004099646.1 HHA domain-containing protein Toxin
Km24235_RS21380 (Km24235_4241) 4546660..4547226 + 567 WP_038424164.1 maltose O-acetyltransferase -
Km24235_RS29025 4547198..4547332 - 135 WP_223226764.1 hypothetical protein -
Km24235_RS21385 (Km24235_4242) 4547353..4547823 + 471 WP_014228205.1 YlaC family protein -
Km24235_RS21390 (Km24235_4243) 4547798..4549252 - 1455 WP_222582473.1 PLP-dependent aminotransferase family protein -
Km24235_RS21395 (Km24235_4244) 4549354..4550052 + 699 WP_014228203.1 GNAT family protein -
Km24235_RS21400 (Km24235_4245) 4550049..4550189 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
Km24235_RS21405 (Km24235_4246) 4550189..4550452 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T212858 WP_004099646.1 NZ_CP081351:4546271-4546489 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T212858 NZ_CP107383:882446-882853 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14350.09 Da        Isoelectric Point: 4.8989

>AT212858 WP_004129911.1 NZ_CP081351:4545870-4546244 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT212858 NZ_CP107383:882199-882465 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HD25

References