Detailed information of TA system TA212849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DhiAT/dnstrm_HI1420(antitoxin)
• Location: 1171450..1172204
• Replicon: chromosome
• Accession: NZ_CP081351
• Organism: Klebsiella michiganensis strain CCRI-24235

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: -
• Locus tag: Km24235_RS05620
• Protein ID: WP_225936001.1
• Coordinates: 1171965..1172204 (-)
• Length: 80 a.a.

Antitoxin (Protein)

• Gene name: dhiA
• Uniprot ID: -
• Locus tag: Km24235_RS05615
• Protein ID: WP_222582219.1
• Coordinates: 1171450..1171953 (-)
• Length: 168 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Km24235_RS05600 (Km24235_1113)	1166992..1167144	+	153	WP_009651623.1	Hok/Gef family protein	-
Km24235_RS05605 (Km24235_1114)	1167264..1169831	-	2568	WP_222582217.1	DNA mismatch repair protein MutS	-
Km24235_RS05610 (Km24235_1115)	1169979..1171430	+	1452	WP_222582218.1	tyrosine-type recombinase/integrase	-
Km24235_RS05615 (Km24235_1116)	1171450..1171953	-	504	WP_222582219.1	addiction module antitoxin	Antitoxin
Km24235_RS05620	1171965..1172204	-	240	WP_225936001.1	DUF4160 domain-containing protein	Toxin
Km24235_RS05625 (Km24235_1117)	1172437..1173048	+	612	WP_222582220.1	hypothetical protein	-
Km24235_RS05630 (Km24235_1118)	1173147..1174226	+	1080	WP_222582221.1	hypothetical protein	-
Km24235_RS28870 (Km24235_1119)	1174416..1174988	-	573	WP_225935982.1	hypothetical protein	-
Km24235_RS05640 (Km24235_1120)	1175435..1176658	+	1224	WP_222582222.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1144940..1200410	55470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (3-67)

Antitoxin

• Uncharacterized protein HI1420 (79-158)

Sequences

Toxin

Protein

• Length: 80 a.a.
• Molecular weight: 9106.73 Da
• Isoelectric Point: 10.1772

>T212849 WP_225936001.1 NZ_CP081351:c1172204-1171965 [Klebsiella michiganensis]
MVGLIFYMYYYDTGKHNSPHVHVKYSSHELIVDINTGVEIVGYLPNKQRKMALLHIKVNKSKLLKMWKLAVNGNNPGHL

Nucleotide

• Length: 240 bp

>T212849 NZ_CP107379:c5054403-5053486 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 168 a.a.
• Molecular weight: 18775.46 Da
• Isoelectric Point: 5.8070

>AT212849 WP_222582219.1 NZ_CP081351:c1171953-1171450 [Klebsiella michiganensis]
MNKIIDVDVIGKFELKLTYENGTVCEVDLYKYNDQPPFTDPKKFVKFGLLPNGNLEWIPNVTISSEALLQRGSNITHDES
IKKTNFANIISMALYDAIIENRPEIIQAALRTAVDKYGTTKIAKNSGTKSRTSIYKSLDENTYVSMSTVVNLAHAVLDLE
EKESILR

Nucleotide

• Length: 504 bp

>AT212849 NZ_CP107379:c5055167-5054409 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA