Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 872584..873241 Replicon chromosome
Accession NZ_CP081351
Organism Klebsiella michiganensis strain CCRI-24235

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag Km24235_RS04220 Protein ID WP_004854060.1
Coordinates 872831..873241 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag Km24235_RS04215 Protein ID WP_004124953.1
Coordinates 872584..872850 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Km24235_RS04200 (Km24235_0832) 867818..868243 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
Km24235_RS04205 (Km24235_0833) 868364..871162 - 2799 WP_085520148.1 transcriptional regulator DagR -
Km24235_RS04210 (Km24235_0834) 871356..872339 - 984 WP_085520149.1 tRNA-modifying protein YgfZ -
Km24235_RS04215 (Km24235_0835) 872584..872850 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
Km24235_RS04220 (Km24235_0836) 872831..873241 + 411 WP_004854060.1 protein YgfX Toxin
Km24235_RS04225 (Km24235_0837) 873250..873771 - 522 WP_014226792.1 flavodoxin FldB -
Km24235_RS04230 (Km24235_0838) 873893..874789 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
Km24235_RS04235 (Km24235_0839) 874812..875525 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
Km24235_RS04240 (Km24235_0840) 875531..877264 + 1734 WP_014226791.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T212848 WP_004854060.1 NZ_CP081351:872831-873241 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T212848 NZ_CP107379:c4897586-4897302 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT212848 WP_004124953.1 NZ_CP081351:872584-872850 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT212848 NZ_CP107379:c4897818-4897576 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References