Detailed information of TA system TA212842

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5121343..5121951
• Replicon: chromosome
• Accession: NZ_CP081346
• Organism: Pseudomonas aeruginosa strain SE5419

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: K5B69_RS24275
• Protein ID: WP_016263850.1
• Coordinates: 5121343..5121690 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: K5B69_RS24280
• Protein ID: WP_003114155.1
• Coordinates: 5121700..5121951 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B69_RS24245 (K5B69_24245)	5116702..5116974	+	273	WP_003120794.1	cysteine-rich CWC family protein	-
K5B69_RS24250 (K5B69_24250)	5116974..5117666	+	693	WP_003098362.1	16S rRNA pseudouridine(516) synthase	-
K5B69_RS24255 (K5B69_24255)	5117802..5118845	+	1044	WP_003098363.1	L,D-transpeptidase	-
K5B69_RS24260 (K5B69_24260)	5118925..5119662	+	738	WP_003085453.1	murein L,D-transpeptidase catalytic domain family protein	-
K5B69_RS24265 (K5B69_24265)	5120114..5121016	+	903	WP_003098365.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
K5B69_RS24275 (K5B69_24275)	5121343..5121690	-	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5B69_RS24280 (K5B69_24280)	5121700..5121951	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
K5B69_RS24285 (K5B69_24285)	5122165..5123148	-	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
K5B69_RS24290 (K5B69_24290)	5123148..5124440	-	1293	WP_003115206.1	hypothetical protein	-
K5B69_RS24295 (K5B69_24295)	5124699..5125961	-	1263	WP_034016890.1	zonular occludens toxin domain-containing protein	-
K5B69_RS24300 (K5B69_24300)	5125963..5126313	-	351	WP_003133726.1	DUF2523 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	5121343..5140571	19228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T212842 WP_016263850.1 NZ_CP081346:c5121690-5121343 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T212842 NZ_CP107379:355319-355666 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT212842 WP_003114155.1 NZ_CP081346:c5121951-5121700 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT212842 NZ_CP107379:355666-355965 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355