Detailed information of TA system TA212839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 2946367..2947588
• Replicon: chromosome
• Accession: NZ_CP081346
• Organism: Pseudomonas aeruginosa strain SE5419

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: K5B69_RS14190
• Protein ID: WP_003102740.1
• Coordinates: 2946367..2946945 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K5B69_RS14195
• Protein ID: WP_003088112.1
• Coordinates: 2947034..2947588 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B69_RS14175 (K5B69_14175)	2941643..2942920	+	1278	WP_003102743.1	TolC family protein	-
K5B69_RS14180 (K5B69_14180)	2942910..2945081	+	2172	WP_179063259.1	type I secretion system permease/ATPase	-
K5B69_RS14185 (K5B69_14185)	2945071..2946258	+	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
K5B69_RS14190 (K5B69_14190)	2946367..2946945	-	579	WP_003102740.1	HD domain-containing protein	Toxin
K5B69_RS14195 (K5B69_14195)	2947034..2947588	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
K5B69_RS14200 (K5B69_14200)	2947746..2949941	-	2196	WP_014603034.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
K5B69_RS14205 (K5B69_14205)	2949946..2950407	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
K5B69_RS14210 (K5B69_14210)	2950738..2951061	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
K5B69_RS14215 (K5B69_14215)	2951074..2951472	+	399	WP_003102731.1	NADH-quinone oxidoreductase subunit A	-
K5B69_RS14220 (K5B69_14220)	2951469..2952020	-	552	WP_003102727.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T212839 WP_003102740.1 NZ_CP081346:c2946945-2946367 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T212839 NZ_CP107377:c4371-4057 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT212839 WP_003088112.1 NZ_CP081346:c2947588-2947034 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT212839 NZ_CP107377:c4637-4359 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Toxin

• AlphaFold DB: A0A0C7CS07