Detailed information of TA system TA212837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 150090..150595
• Replicon: chromosome
• Accession: NZ_CP081346
• Organism: Pseudomonas aeruginosa strain SE5419

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: K5B69_RS00680
• Protein ID: WP_003083773.1
• Coordinates: 150090..150371 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: K5B69_RS00685
• Protein ID: WP_003083775.1
• Coordinates: 150368..150595 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B69_RS00655 (K5B69_00655)	145341..146690	+	1350	WP_003142411.1	C4-dicarboxylate transporter DctA	-
K5B69_RS00660 (K5B69_00660)	146739..147425	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
K5B69_RS00665 (K5B69_00665)	147526..148260	+	735	WP_004346926.1	GntR family transcriptional regulator	-
K5B69_RS00670 (K5B69_00670)	148440..148850	+	411	WP_003101225.1	aegerolysin family protein	-
K5B69_RS00675 (K5B69_00675)	148882..149790	-	909	WP_003083769.1	LysR family transcriptional regulator	-
K5B69_RS00680 (K5B69_00680)	150090..150371	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
K5B69_RS00685 (K5B69_00685)	150368..150595	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
K5B69_RS00690 (K5B69_00690)	150771..151391	-	621	WP_003101226.1	hypothetical protein	-
K5B69_RS00695 (K5B69_00695)	151492..151992	+	501	WP_003112629.1	LEA type 2 family protein	-
K5B69_RS00700 (K5B69_00700)	152065..152406	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
K5B69_RS00705 (K5B69_00705)	152488..153915	-	1428	WP_003083784.1	GABA permease	-
K5B69_RS00710 (K5B69_00710)	154084..155577	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T212837 WP_003083773.1 NZ_CP081346:c150371-150090 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T212837 NZ_CP107373:171896-172339 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT212837 WP_003083775.1 NZ_CP081346:c150595-150368 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT212837 NZ_CP107373:171669-171899 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGCTCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9