Detailed information of TA system TA212835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4776690..4777948
• Replicon: chromosome
• Accession: NZ_CP081345
• Organism: Pseudomonas aeruginosa strain F291007

Toxin (Protein)

• Gene name: -
• Uniprot ID: V6AJ34
• Locus tag: K5B02_RS22240
• Protein ID: WP_003086331.1
• Coordinates: 4776690..4777445 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: K5B02_RS22245
• Protein ID: WP_003086329.1
• Coordinates: 4777580..4777948 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B02_RS22230 (K5B02_22230)	4771861..4774404	+	2544	WP_003086335.1	acyl-homoserine-lactone acylase QuiP	-
K5B02_RS22235 (K5B02_22235)	4774559..4775920	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
K5B02_RS22240 (K5B02_22240)	4776690..4777445	-	756	WP_003086331.1	RES family NAD+ phosphorylase	Toxin
K5B02_RS22245 (K5B02_22245)	4777580..4777948	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
K5B02_RS22250 (K5B02_22250)	4778021..4779307	-	1287	WP_003086327.1	FAD-binding oxidoreductase	-
K5B02_RS22255 (K5B02_22255)	4779363..4780952	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
K5B02_RS22260 (K5B02_22260)	4781031..4781507	+	477	WP_003086322.1	hypothetical protein	-
K5B02_RS22265 (K5B02_22265)	4781510..4781785	-	276	WP_023098713.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28236.34 Da
• Isoelectric Point: 7.7294

>T212835 WP_003086331.1 NZ_CP081345:c4777445-4776690 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T212835 NZ_CP107373:56134-56445 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT212835 WP_003086329.1 NZ_CP081345:c4777948-4777580 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT212835 NZ_CP107373:56442-56861 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Structures

Toxin

• AlphaFold DB: V6AJ34

Antitoxin

• AlphaFold DB: V6AJU7