Detailed information of TA system TA212834

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4213571..4214613
• Replicon: chromosome
• Accession: NZ_CP081345
• Organism: Pseudomonas aeruginosa strain F291007

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: K5B02_RS19540
• Protein ID: WP_003153636.1
• Coordinates: 4213571..4214146 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: K5B02_RS19545
• Protein ID: WP_003050245.1
• Coordinates: 4214143..4214613 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B02_RS19515 (K5B02_19515)	4210009..4210734	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
K5B02_RS19520 (K5B02_19520)	4210773..4211675	-	903	WP_003090158.1	CBASS oligonucleotide cyclase	-
K5B02_RS19525 (K5B02_19525)	4211675..4212175	-	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
K5B02_RS19530 (K5B02_19530)	4212172..4212642	-	471	WP_063305693.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
K5B02_RS19535 (K5B02_19535)	4212639..4213553	-	915	WP_016852809.1	AAA family ATPase	-
K5B02_RS19540 (K5B02_19540)	4213571..4214146	-	576	WP_003153636.1	PIN domain-containing protein	Toxin
K5B02_RS19545 (K5B02_19545)	4214143..4214613	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
K5B02_RS19550 (K5B02_19550)	4214817..4215200	+	384	WP_003090167.1	RAQPRD family integrative conjugative element protein	-
K5B02_RS19555 (K5B02_19555)	4215197..4215430	+	234	WP_006226027.1	TIGR03758 family integrating conjugative element protein	-
K5B02_RS19560 (K5B02_19560)	4215447..4215806	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
K5B02_RS19565 (K5B02_19565)	4215818..4216216	+	399	WP_003050133.1	TIGR03750 family conjugal transfer protein	-
K5B02_RS19570 (K5B02_19570)	4216213..4216905	+	693	WP_003090182.1	TIGR03746 family integrating conjugative element protein	-
K5B02_RS19575 (K5B02_19575)	4216902..4217813	+	912	WP_006226028.1	TIGR03749 family integrating conjugative element protein	-
K5B02_RS19580 (K5B02_19580)	4217803..4219221	+	1419	WP_006226029.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4165175..4289188	124013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21629.78 Da
• Isoelectric Point: 5.9995

>T212834 WP_003153636.1 NZ_CP081345:c4214146-4213571 [Pseudomonas aeruginosa]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPNDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIDVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T212834 NZ_CP107373:18106-18576 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT212834 WP_003050245.1 NZ_CP081345:c4214613-4214143 [Pseudomonas aeruginosa]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT212834 NZ_CP107373:17666-18109 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: I3TV68