Detailed information of TA system TA212833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3264523..3265744
• Replicon: chromosome
• Accession: NZ_CP081345
• Organism: Pseudomonas aeruginosa strain F291007

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: V6AG16
• Locus tag: K5B02_RS15580
• Protein ID: WP_003088107.1
• Coordinates: 3264523..3265101 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: K5B02_RS15585
• Protein ID: WP_003088112.1
• Coordinates: 3265190..3265744 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B02_RS15565 (K5B02_15565)	3259799..3261076	+	1278	WP_003088104.1	TolC family protein	-
K5B02_RS15570 (K5B02_15570)	3261066..3263237	+	2172	WP_003088105.1	type I secretion system permease/ATPase	-
K5B02_RS15575 (K5B02_15575)	3263227..3264414	+	1188	WP_003088106.1	HlyD family efflux transporter periplasmic adaptor subunit	-
K5B02_RS15580 (K5B02_15580)	3264523..3265101	-	579	WP_003088107.1	HD domain-containing protein	Toxin
K5B02_RS15585 (K5B02_15585)	3265190..3265744	-	555	WP_003088112.1	helix-turn-helix transcriptional regulator	Antitoxin
K5B02_RS15590 (K5B02_15590)	3265902..3268097	-	2196	WP_003088114.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
K5B02_RS15595 (K5B02_15595)	3268102..3268563	-	462	WP_003088117.1	(2Fe-2S)-binding protein	-
K5B02_RS15600 (K5B02_15600)	3268894..3269217	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
K5B02_RS15605 (K5B02_15605)	3269230..3269628	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
K5B02_RS15610 (K5B02_15610)	3269625..3270176	-	552	WP_023098678.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-187)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21885.74 Da
• Isoelectric Point: 5.3655

>T212833 WP_003088107.1 NZ_CP081345:c3265101-3264523 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDAKTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T212833 NZ_CP107372:c5365914-5365531 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT212833 WP_003088112.1 NZ_CP081345:c3265744-3265190 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT212833 NZ_CP107372:c5366156-5365914 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: V6AG16