Detailed information of TA system TA212831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 183904..184409
• Replicon: chromosome
• Accession: NZ_CP081345
• Organism: Pseudomonas aeruginosa strain F291007

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: K5B02_RS00800
• Protein ID: WP_003083773.1
• Coordinates: 183904..184185 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: K5B02_RS00805
• Protein ID: WP_003083775.1
• Coordinates: 184182..184409 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5B02_RS00775 (K5B02_00775)	179155..180504	+	1350	WP_003137006.1	C4-dicarboxylate transporter DctA	-
K5B02_RS00780 (K5B02_00780)	180553..181239	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
K5B02_RS00785 (K5B02_00785)	181340..182074	+	735	WP_003083764.1	GntR family transcriptional regulator	-
K5B02_RS00790 (K5B02_00790)	182254..182664	+	411	WP_003110659.1	aegerolysin family protein	-
K5B02_RS00795 (K5B02_00795)	182696..183604	-	909	WP_003083769.1	LysR family transcriptional regulator	-
K5B02_RS00800 (K5B02_00800)	183904..184185	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
K5B02_RS00805 (K5B02_00805)	184182..184409	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
K5B02_RS00810 (K5B02_00810)	184585..185205	-	621	WP_003101226.1	hypothetical protein	-
K5B02_RS00815 (K5B02_00815)	185306..185806	+	501	WP_003083778.1	LEA type 2 family protein	-
K5B02_RS00820 (K5B02_00820)	185879..186220	+	342	WP_014603467.1	zinc ribbon domain-containing protein YjdM	-
K5B02_RS00825 (K5B02_00825)	186302..187729	-	1428	WP_003083784.1	GABA permease	-
K5B02_RS00830 (K5B02_00830)	187898..189391	-	1494	WP_003083788.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T212831 WP_003083773.1 NZ_CP081345:c184185-183904 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T212831 NZ_CP107372:c4885580-4885296 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT212831 WP_003083775.1 NZ_CP081345:c184409-184182 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT212831 NZ_CP107372:c4885812-4885570 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9