Detailed information of TA system TA212830

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 119653..120560
• Replicon: plasmid plas2
• Accession: NZ_CP081344
• Organism: Pantoea ananatis strain TZ39

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K4732_RS21880
• Protein ID: WP_047713814.1
• Coordinates: 120093..120560 (+)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: K4732_RS21875
• Protein ID: WP_014598335.1
• Coordinates: 119653..120096 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4732_RS21845 (K4732_21845)	114791..115177	-	387	WP_013027868.1	pyrimidine utilization protein C	-
K4732_RS21850 (K4732_21850)	115203..115955	-	753	WP_196247056.1	pyrimidine utilization protein B	-
K4732_RS21855 (K4732_21855)	115952..117043	-	1092	WP_047713811.1	pyrimidine utilization protein A	-
K4732_RS21860 (K4732_21860)	117442..118131	+	690	WP_014598333.1	HTH-type transcriptional regulator RutR	-
K4732_RS21865 (K4732_21865)	118207..118902	-	696	WP_221612112.1	hypothetical protein	-
K4732_RS21870 (K4732_21870)	119042..119266	-	225	WP_221612113.1	KTSC domain-containing protein	-
K4732_RS21875 (K4732_21875)	119653..120096	+	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
K4732_RS21880 (K4732_21880)	120093..120560	+	468	WP_047713814.1	RES family NAD+ phosphorylase	Toxin
K4732_RS21885 (K4732_21885)	121205..122599	+	1395	WP_028722484.1	flagellar filament capping protein FliD	-
K4732_RS21890 (K4732_21890)	123282..124217	+	936	WP_028722485.1	flagellin	-
K4732_RS21895 (K4732_21895)	124279..124851	-	573	WP_028722486.1	NADPH-dependent FMN reductase	-
K4732_RS21900 (K4732_21900)	124939..125172	+	234	WP_028722487.1	DUF333 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vipA/tssB / vipB/tssC / tssG / fliC	1..276579	276579

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17234.72 Da
• Isoelectric Point: 5.1570

>T212830 WP_047713814.1 NZ_CP081344:120093-120560 [Pantoea ananatis]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPDDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFNAIVRQAVNIPFLFDVRFSRQA

Nucleotide

• Length: 468 bp

>T212830 NZ_CP107372:c4783214-4782681 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT212830 WP_014598335.1 NZ_CP081344:119653-120096 [Pantoea ananatis]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT212830 NZ_CP107372:c4783491-4783225 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA