Detailed information of TA system TA212829

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 11866..12509
• Replicon: plasmid plas1
• Accession: NZ_CP081343
• Organism: Pantoea ananatis strain TZ39

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K4732_RS20775
• Protein ID: WP_221612008.1
• Coordinates: 11866..12282 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: K4732_RS20780
• Protein ID: WP_047596145.1
• Coordinates: 12279..12509 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4732_RS20765 (K4732_20765)	8591..10294	+	1704	WP_221612007.1	HNH endonuclease	-
K4732_RS22935	10318..10449	-	132	WP_260412027.1	hypothetical protein	-
K4732_RS20775 (K4732_20775)	11866..12282	-	417	WP_221612008.1	type II toxin-antitoxin system VapC family toxin	Toxin
K4732_RS20780 (K4732_20780)	12279..12509	-	231	WP_047596145.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K4732_RS20785 (K4732_20785)	13068..13427	+	360	WP_047714313.1	hypothetical protein	-
K4732_RS20790 (K4732_20790)	13481..13984	+	504	WP_221612009.1	hypothetical protein	-
K4732_RS20795 (K4732_20795)	14287..15063	+	777	WP_221612010.1	site-specific integrase	-
K4732_RS20800 (K4732_20800)	15176..15802	-	627	WP_221612011.1	transposase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135135	135135

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15141.51 Da
• Isoelectric Point: 6.7054

>T212829 WP_221612008.1 NZ_CP081343:c12282-11866 [Pantoea ananatis]
VNRTYMLDTCICSFIMREQPAAVIERLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVEEFCARLDAVLPWDRA
AVDATTEIKVVLRLAGTPIGPNDTAIAGHAISAGAVLVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T212829 NZ_CP107372:4186644-4186862 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8765.88 Da
• Isoelectric Point: 4.3775

>AT212829 WP_047596145.1 NZ_CP081343:c12509-12279 [Pantoea ananatis]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT212829 NZ_CP107372:4186243-4186617 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG