Detailed information of TA system TA021282

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 111429..112087
• Replicon: plasmid pXCV183
• Accession: NC_007507
• Organism: Xanthomonas campestris pv. vesicatoria str. 85-10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q8PRH3
• Locus tag: IS_RS00910
• Protein ID: WP_011053002.1
• Coordinates: 111429..111830 (-)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q3C002
• Locus tag: IS_RS00915
• Protein ID: WP_005917852.1
• Coordinates: 111827..112087 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS_RS00895	108441..109667	-	1227	WP_011345704.1	replication protein	-
IS_RS00900	109947..111134	-	1188	WP_011053001.1	zeta toxin family protein	-
IS_RS00905	111141..111404	-	264	WP_005917846.1	hypothetical protein	-
IS_RS00910	111429..111830	-	402	WP_011053002.1	type II toxin-antitoxin system VapC family toxin	Toxin
IS_RS00915	111827..112087	-	261	WP_005917852.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
IS_RS00920	112277..112843	+	567	WP_011345705.1	recombinase family protein	-
IS_RS24000	113115..114452	-	1338	WP_011037255.1	avirulence protein	-
IS_RS26465	114543..115613	-	1071	WP_011345706.1	tyrosine phosphatase	-
IS_RS00925	115889..116311	-	423	Protein_114	resolvase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..182572	182572
	-	inside	IScluster/Tn	-	-	99654..127168	27514

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• SpoVT-AbrB domain (8-52)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 14627.05 Da
• Isoelectric Point: 7.2785

>T21282 WP_011053002.1 NC_007507:c111830-111429 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MKRYMLDTNTVSHLVKSHPAVSRRVIEVPMTALCMSAITGGELMFGLAKVPDAKRLQQAVMELLRRVDVLPWDGAVMERY
GSVRADLEKQGKALGSLDMLIAAHALETDSVLVTNDAAFSRVVGLTVEDWTRS

Nucleotide

• Length: 402 bp

>T21282 NC_007507:c111830-111429 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGAAGCGGTACATGCTTGATACCAACACCGTGAGCCATCTCGTCAAGAGCCATCCGGCTGTCTCTCGACGGGTCATTGA
AGTGCCTATGACTGCGCTATGTATGTCCGCAATTACCGGTGGGGAGCTGATGTTCGGCTTGGCTAAGGTGCCAGACGCCA
AACGCCTTCAGCAAGCCGTCATGGAACTCCTGCGGCGCGTGGATGTGCTGCCTTGGGATGGCGCGGTAATGGAGCGTTAC
GGAAGCGTAAGAGCGGACCTAGAAAAGCAAGGTAAAGCGCTTGGGTCGCTCGACATGCTTATCGCAGCACATGCACTGGA
AACGGATTCTGTCCTCGTTACCAATGACGCCGCATTTAGTCGTGTTGTTGGTTTGACCGTGGAAGATTGGACACGCAGCT
AA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9770.95 Da
• Isoelectric Point: 5.0941

>AT21282 WP_005917852.1 NC_007507:c112087-111827 [Xanthomonas campestris pv. vesicatoria str. 85-10]
VSQVAKLFINGRSQAVRLPAAYRFTEKEVFIRQDPETGDVILSRKPATWEDFFIALKSADVPANFLDAAERNQGTQDRDP
FAGWRE

Nucleotide

• Length: 261 bp

>AT21282 NC_007507:c112087-111827 [Xanthomonas campestris pv. vesicatoria str. 85-10]
GTGAGCCAAGTTGCCAAGCTGTTCATCAATGGCCGCAGCCAGGCAGTCCGCTTGCCTGCCGCCTATCGCTTTACCGAGAA
AGAGGTTTTCATTCGCCAAGACCCGGAGACCGGTGACGTCATCCTGTCTCGGAAGCCTGCAACGTGGGAGGACTTCTTTA
TCGCACTCAAGAGCGCAGATGTGCCTGCTAATTTTCTAGATGCCGCCGAGCGCAATCAGGGCACGCAGGACCGTGATCCT
TTTGCGGGATGGCGCGAATGA

Structures

Toxin

• AlphaFold DB: Q8PRH3

Antitoxin

• AlphaFold DB: Q3C002