Detailed information of TA system TA212817

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 100701..101602
• Replicon: plasmid pCCRI24236-1
• Accession: NZ_CP081315
• Organism: Citrobacter farmeri strain CCRI-24236

Toxin (Protein)

• Gene name: -
• Uniprot ID: S1EZ83
• Locus tag: Cf24236_RS24475
• Protein ID: WP_008786589.1
• Coordinates: 101144..101602 (+)
• Length: 153 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: Cf24236_RS24470
• Protein ID: WP_008786588.1
• Coordinates: 100701..101147 (+)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Cf24236_RS24435 (Cf24236_5116)	96064..96315	-	252	WP_024196080.1	hypothetical protein	-
Cf24236_RS24440 (Cf24236_5117)	96737..97579	-	843	WP_007372307.1	HNH endonuclease signature motif containing protein	-
Cf24236_RS24445 (Cf24236_5119)	98147..98311	-	165	WP_007372306.1	hypothetical protein	-
Cf24236_RS24450 (Cf24236_5120)	98598..99041	-	444	WP_007372305.1	hypothetical protein	-
Cf24236_RS24455 (Cf24236_5121)	99101..99379	-	279	WP_007372304.1	hypothetical protein	-
Cf24236_RS24460 (Cf24236_5122)	99351..100193	-	843	WP_007372303.1	hypothetical protein	-
Cf24236_RS24465 (Cf24236_5123)	100235..100552	-	318	WP_016241555.1	hypothetical protein	-
Cf24236_RS24470 (Cf24236_5124)	100701..101147	+	447	WP_008786588.1	DUF2384 domain-containing protein	Antitoxin
Cf24236_RS24475 (Cf24236_5125)	101144..101602	+	459	WP_008786589.1	RES family NAD+ phosphorylase	Toxin
Cf24236_RS24480 (Cf24236_5126)	101646..101876	+	231	WP_007372299.1	hypothetical protein	-
Cf24236_RS24485 (Cf24236_5127)	101944..102210	-	267	WP_007372298.1	hypothetical protein	-
Cf24236_RS24490 (Cf24236_5128)	102260..102814	-	555	WP_007372297.1	hypothetical protein	-
Cf24236_RS24495 (Cf24236_5129)	102973..103314	-	342	WP_007372296.1	hypothetical protein	-
Cf24236_RS24500 (Cf24236_5130)	103433..103876	-	444	WP_007372295.1	hypothetical protein	-
Cf24236_RS24505 (Cf24236_5131)	104114..104452	+	339	WP_007372294.1	hypothetical protein	-
Cf24236_RS24510 (Cf24236_5132)	104815..105141	-	327	WP_007372293.1	hypothetical protein	-
Cf24236_RS24515 (Cf24236_5133)	105225..105623	-	399	WP_222563844.1	hypothetical protein	-
Cf24236_RS24520 (Cf24236_5134)	105634..105921	-	288	WP_016241556.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..198299	198299

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (95-145)

Sequences

Toxin

Protein

• Length: 153 a.a.
• Molecular weight: 17005.43 Da
• Isoelectric Point: 4.5730

>T212817 WP_008786589.1 NZ_CP081315:101144-101602 [Citrobacter farmeri]
VILYRLTKTRYLMTAWSGLGAKETGGRWNSVGTAMVYLSETASLTMLETLVHIHAPQLLDDFTLLSLDVPDDQIQTFDMS
RLPDNWASEDAPAELALYGDNWAESGSSIALRIPSALSPVEFNYLLNPAHPELFDLMATVKKIPFRFDSRLK

Nucleotide

• Length: 459 bp

>T212817 NZ_CP107367:c5043966-5043049 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16689.02 Da
• Isoelectric Point: 6.9812

>AT212817 WP_008786588.1 NZ_CP081315:100701-101147 [Citrobacter farmeri]
MTMKVFSPDVTKTNQHCLWQVAGLDNDGIALMDKINHGLDGTVAKHISEWANITPSELRKMSGIPNTTFNRSIKDRFTAD
QSERLVRIIRVIERAVELFEGDKEAAHKWLNEANRGLSWKSPAELVSSETGALEVMRLITRIEHGVYS

Nucleotide

• Length: 447 bp

>AT212817 NZ_CP107367:c5044730-5043972 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EZ83