Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 71493..72019 Replicon plasmid pCCRI24236-1
Accession NZ_CP081315
Organism Citrobacter farmeri strain CCRI-24236

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag Cf24236_RS24295 Protein ID WP_000323025.1
Coordinates 71493..71780 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag Cf24236_RS24300 Protein ID WP_000534858.1
Coordinates 71780..72019 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Cf24236_RS24280 (Cf24236_5084) 66861..70238 - 3378 WP_007372331.1 hypothetical protein -
Cf24236_RS24285 (Cf24236_5085) 70442..70687 - 246 WP_032951184.1 hypothetical protein -
Cf24236_RS24290 (Cf24236_5086) 70736..71395 - 660 WP_008786574.1 hypothetical protein -
Cf24236_RS24295 (Cf24236_5087) 71493..71780 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
Cf24236_RS24300 (Cf24236_5088) 71780..72019 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
Cf24236_RS26390 72044..72148 + 105 Protein_83 protein YdfV -
Cf24236_RS24305 (Cf24236_5089) 72282..73205 - 924 WP_000167917.1 cation diffusion facilitator family transporter -
Cf24236_RS24310 (Cf24236_5090) 73405..73977 - 573 WP_001515348.1 cytochrome b/b6 domain-containing protein -
Cf24236_RS24315 (Cf24236_5091) 74453..75691 - 1239 WP_003030308.1 IS110 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..198299 198299


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T212816 WP_000323025.1 NZ_CP081315:c71780-71493 [Citrobacter farmeri]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T212816 NZ_CP107367:c4885949-4885665 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT212816 WP_000534858.1 NZ_CP081315:c72019-71780 [Citrobacter farmeri]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT212816 NZ_CP107367:c4886181-4885939 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References