Detailed information of TA system TA212815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4837227..4837852
• Replicon: chromosome
• Accession: NZ_CP081314
• Organism: Citrobacter farmeri strain CCRI-24236

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Cf24236_RS23000
• Protein ID: WP_195297256.1
• Coordinates: 4837227..4837613 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: Cf24236_RS23005
• Protein ID: WP_225935005.1
• Coordinates: 4837613..4837852 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Cf24236_RS22980 (Cf24236_4589)	4832580..4833917	+	1338	WP_195619895.1	VWA domain-containing protein	-
Cf24236_RS22985 (Cf24236_4590)	4833935..4835431	+	1497	WP_216526260.1	HAMP domain-containing sensor histidine kinase	-
Cf24236_RS22990 (Cf24236_4591)	4835436..4836122	+	687	WP_195506347.1	response regulator transcription factor	-
Cf24236_RS22995 (Cf24236_4592)	4836267..4837196	+	930	WP_195297257.1	formate dehydrogenase accessory protein FdhE	-
Cf24236_RS23000 (Cf24236_4593)	4837227..4837613	-	387	WP_195297256.1	type II toxin-antitoxin system VapC family toxin	Toxin
Cf24236_RS23005 (Cf24236_4594)	4837613..4837852	-	240	WP_225935005.1	CopG family transcriptional regulator	Antitoxin
Cf24236_RS23010 (Cf24236_4595)	4838064..4838996	+	933	WP_216526261.1	alpha/beta hydrolase	-
Cf24236_RS23015 (Cf24236_4596)	4839014..4839955	-	942	WP_195297253.1	fatty acid biosynthesis protein FabY	-
Cf24236_RS23020 (Cf24236_4597)	4840000..4840437	-	438	WP_216526262.1	D-aminoacyl-tRNA deacylase	-
Cf24236_RS23025 (Cf24236_4598)	4840434..4841306	-	873	WP_042325452.1	virulence factor BrkB family protein	-
Cf24236_RS23030 (Cf24236_4599)	4841300..4841899	-	600	WP_216526263.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-46)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14561.91 Da
• Isoelectric Point: 10.1327

>T212815 WP_195297256.1 NZ_CP081314:c4837613-4837227 [Citrobacter farmeri]
MHKKPVLFDTNILIDLFSGHQEAQRALEAFPPQNAISLITWMEVMVGAKKYHQEYRTRVALSAFNIIGVSPEIAERSVNL
RQEYRMKLPDAIILATAQIHRFALVTRNTRDFAGIPGVITPYQLQTGR

Nucleotide

• Length: 387 bp

>T212815 NZ_CP107367:c4783457-4782924 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9181.41 Da
• Isoelectric Point: 4.2075

>AT212815 WP_225935005.1 NZ_CP081314:c4837852-4837613 [Citrobacter farmeri]
MAGMDMGRILLDLSDEAIKRLDDLKQLRNLPRAELLREAVEQYLERQGQAETMISNALGIWQGCEEDGVEYQKTLREEW

Nucleotide

• Length: 240 bp

>AT212815 NZ_CP107367:c4783734-4783468 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA