Detailed information of TA system TA212811

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4131643..4132297
• Replicon: chromosome
• Accession: NZ_CP081314
• Organism: Citrobacter farmeri strain CCRI-24236

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: Cf24236_RS19665
• Protein ID: WP_125369695.1
• Coordinates: 4131643..4132050 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A0F6TWP9
• Locus tag: Cf24236_RS19670
• Protein ID: WP_042322123.1
• Coordinates: 4132031..4132297 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Cf24236_RS19645 (Cf24236_3919)	4127571..4129304	-	1734	WP_195504947.1	single-stranded-DNA-specific exonuclease RecJ	-
Cf24236_RS19650 (Cf24236_3920)	4129310..4130023	-	714	WP_195296564.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
Cf24236_RS19655 (Cf24236_3921)	4130047..4130943	-	897	WP_195296836.1	site-specific tyrosine recombinase XerD	-
Cf24236_RS19660 (Cf24236_3922)	4131044..4131565	+	522	WP_195296563.1	flavodoxin FldB	-
Cf24236_RS19665 (Cf24236_3923)	4131643..4132050	-	408	WP_125369695.1	protein YgfX	Toxin
Cf24236_RS19670 (Cf24236_3924)	4132031..4132297	-	267	WP_042322123.1	FAD assembly factor SdhE	Antitoxin
Cf24236_RS19675 (Cf24236_3925)	4132554..4133534	+	981	WP_195619699.1	tRNA-modifying protein YgfZ	-
Cf24236_RS19680 (Cf24236_3926)	4133697..4134356	-	660	WP_042322119.1	hemolysin III family protein	-
Cf24236_RS19685 (Cf24236_3927)	4134519..4134830	-	312	WP_195296561.1	N(4)-acetylcytidine aminohydrolase	-
Cf24236_RS19690 (Cf24236_3928)	4134883..4135611	+	729	WP_216525506.1	MurR/RpiR family transcriptional regulator	-
Cf24236_RS19695 (Cf24236_3929)	4135731..4137164	+	1434	WP_216525496.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15904.87 Da
• Isoelectric Point: 11.3523

>T212811 WP_125369695.1 NZ_CP081314:c4132050-4131643 [Citrobacter farmeri]
VVLWQSDLRVSWRAQWLSLLLHGLVAAAILLMPWPLSYTPLWLILLSLVVFDCVRSQRRINARQGEVKLLMDGRLRWQGQ
DWHIVSAPWMIKTGMMLRLRSETGKRQHLWLAADSMDEAEWRDLRRLMMQKSTLG

Nucleotide

• Length: 408 bp

>T212811 NZ_CP107367:881882-882289 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10544.06 Da
• Isoelectric Point: 5.1496

>AT212811 WP_042322123.1 NZ_CP081314:c4132297-4132031 [Citrobacter farmeri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEQMVRLIQTRN
RDRGPVAI

Nucleotide

• Length: 267 bp

>AT212811 NZ_CP107367:881635-881901 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A0F6TWP9