Detailed information of TA system TA021281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 19160..19701
• Replicon: plasmid pXCV38
• Accession: NC_007506
• Organism: Xanthomonas campestris pv. vesicatoria str. 85-10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IS_RS00255
• Protein ID: WP_007968778.1
• Coordinates: 19160..19486 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7W9EDN7
• Locus tag: IS_RS00260
• Protein ID: WP_033478260.1
• Coordinates: 19483..19701 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS_RS00240	14326..14523	+	198	WP_011345595.1	hypothetical protein	-
IS_RS00245	14527..16101	+	1575	WP_011345596.1	type IV secretion system DNA-binding domain-containing protein	-
IS_RS00250	16115..19090	+	2976	WP_011345597.1	conjugative relaxase	-
IS_RS00255	19160..19486	-	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IS_RS00260	19483..19701	-	219	WP_033478260.1	antitoxin MazE family protein	Antitoxin
IS_RS00265	19838..20254	-	417	WP_017159868.1	hypothetical protein	-
IS_RS00270	20308..20721	-	414	WP_041854931.1	hypothetical protein	-
IS_RS00275	20732..21307	-	576	WP_041854932.1	phospholipase D family protein	-
IS_RS00280	21350..21937	-	588	WP_011345600.1	hypothetical protein	-
IS_RS00285	21983..22870	-	888	WP_041854937.1	lytic transglycosylase domain-containing protein	-
IS_RS00290	22881..23903	-	1023	WP_007968768.1	P-type DNA transfer ATPase VirB11	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38116	38116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T21281 WP_007968778.1 NC_007506:c19486-19160 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T21281 NC_007506:c19486-19160 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGATGCGAGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTCATCCAGGCCAA
CCAGTTTGACGAACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTTGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGCCTGCTGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
ATAGGTCGGGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8476.58 Da
• Isoelectric Point: 5.0742

>AT21281 WP_033478260.1 NC_007506:c19701-19483 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT21281 NC_007506:c19701-19483 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGTGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGAGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7W9EDN7