Detailed information of TA system TA021280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 2729..3264
• Replicon: plasmid pXCV38
• Accession: NC_007506
• Organism: Xanthomonas campestris pv. vesicatoria str. 85-10

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3C0B3
• Locus tag: IS_RS00145
• Protein ID: WP_007974210.1
• Coordinates: 2729..3049 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q3C0B2
• Locus tag: IS_RS00150
• Protein ID: WP_011345582.1
• Coordinates: 3049..3264 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IS_RS00135	407..736	+	330	WP_007967027.1	hypothetical protein	-
IS_RS00140	1242..2060	+	819	WP_011345581.1	hypothetical protein	-
IS_RS00145	2729..3049	-	321	WP_007974210.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IS_RS00150	3049..3264	-	216	WP_011345582.1	antitoxin MazE family protein	Antitoxin
IS_RS00155	3510..3836	-	327	WP_007974207.1	hypothetical protein	-
IS_RS00160	3833..4459	-	627	WP_007974205.1	ParA family protein	-
IS_RS00170	4935..5621	+	687	WP_007968793.1	DNA-binding protein	-
IS_RS00175	5780..6742	+	963	WP_011345584.1	recombinase family protein	-
IS_RS00180	6829..7059	+	231	WP_046935364.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38116	38116

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11571.48 Da
• Isoelectric Point: 6.2890

>T21280 WP_007974210.1 NC_007506:c3049-2729 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MQRGDLVTVSLQGDYGKPRPALVVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Nucleotide

• Length: 321 bp

>T21280 NC_007506:c3049-2729 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGCAGCGGGGCGACCTTGTGACGGTTTCATTGCAGGGCGACTATGGCAAACCACGGCCCGCCTTGGTCGTTCAATCCGA
CTTGCTCACCGACCTGGAAAGCGTCGTGCTGTGCCCGGTGACAAGCGACCTGCGCAATGCCGCGTTCCGCGTCACCGTGG
AACCGAACCCGGCCAACGGGCTGCGCGCGCTCTCGCAGGTCATGGTGGACAAGCTCTCAACGCTGCCTCGCACCAAGATC
AGCGAACCGTTTGGCCGCCTCGATGATGAGCGTATGAAAGCGATAGACAGGGCGCTCCTGCTGGTCGTTGGCGTTATCTG
A

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8190.20 Da
• Isoelectric Point: 7.2801

>AT21280 WP_011345582.1 NC_007506:c3264-3049 [Xanthomonas campestris pv. vesicatoria str. 85-10]
MSSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFAAQVRKQCQNLKGDPAETEVLRFTEEAATHVEGWE

Nucleotide

• Length: 216 bp

>AT21280 NC_007506:c3264-3049 [Xanthomonas campestris pv. vesicatoria str. 85-10]
ATGTCATCAATTCACGAATCAAAGGTAGCCCGGCACCGCGAGCGGATGCGCGCTGCGGGGCTACGGCCTGTCCAGTTTTG
GGTGCCCGACACACGCTCTCCCGAGTTCGCGGCGCAAGTGCGCAAGCAGTGCCAAAACCTGAAGGGCGACCCGGCAGAAA
CCGAGGTTTTGCGCTTCACCGAAGAGGCCGCAACGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: Q3C0B3

Antitoxin

• AlphaFold DB: Q3C0B2