Detailed information of TA system TA212775

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1344544..1345169
• Replicon: chromosome
• Accession: NZ_CP081306
• Organism: Escherichia coli strain Ec1079

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K5A55_RS06540
• Protein ID: WP_000911330.1
• Coordinates: 1344771..1345169 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: K5A55_RS06535
• Protein ID: WP_000450524.1
• Coordinates: 1344544..1344771 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5A55_RS06510 (1340347)	1340347..1340817	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
K5A55_RS06515 (1340817)	1340817..1341389	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
K5A55_RS06520 (1341535)	1341535..1342413	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
K5A55_RS06525 (1342430)	1342430..1343464	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
K5A55_RS06530 (1343677)	1343677..1344390	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
K5A55_RS06535 (1344544)	1344544..1344771	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
K5A55_RS06540 (1344771)	1344771..1345169	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
K5A55_RS06545 (1345316)	1345316..1346179	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
K5A55_RS06550 (1346194)	1346194..1348209	+	2016	WP_000829323.1	tRNA cytosine(34) acetyltransferase TmcA	-
K5A55_RS06555 (1348283)	1348283..1348624	+	342	Protein_1279	esterase	-
K5A55_RS06560 (1348706)	1348706..1349452	-	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T212775 WP_000911330.1 NZ_CP081306:1344771-1345169 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T212775 NZ_CP107353:255550-255966 [Klebsiella pneumoniae]
GTGAACAAAATGTATATGCTCGACACGAATATCTGCTCTTTCATCATGCGCGAGCAGCCGGAAGCAGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCACGTTCAGCTGGTTGACGCATTCTGTGAGCGACTCGATGCCGTCCTGCCCTGGGATCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCGCTGCGCCTCGCCGGAACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCACGCCATCGCCGCCGGCGCCGTGCTGGTGACGAACAATGTGCGGGAATTTGAGCGGGTGCCAGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT212775 WP_000450524.1 NZ_CP081306:1344544-1344771 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT212775 NZ_CP107353:255323-255553 [Klebsiella pneumoniae]
ATGAGAACCGTATCCATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGTATTATCCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTCG
AAAAAGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGGGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33