Detailed information of TA system TA212765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 510587..511224
• Replicon: chromosome
• Accession: NZ_CP081304
• Organism: Bacillus velezensis strain CL-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: K4L72_RS02450
• Protein ID: WP_003156187.1
• Coordinates: 510874..511224 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: K4L72_RS02445
• Protein ID: WP_003156188.1
• Coordinates: 510587..510868 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4L72_RS02425 (506952)	506952..507551	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
K4L72_RS02430 (507644)	507644..508009	+	366	WP_014304402.1	holo-ACP synthase	-
K4L72_RS02435 (508174)	508174..509181	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
K4L72_RS02440 (509298)	509298..510467	+	1170	WP_003156189.1	alanine racemase	-
K4L72_RS02445 (510587)	510587..510868	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
K4L72_RS02450 (510874)	510874..511224	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
K4L72_RS02455 (511342)	511342..512163	+	822	WP_014304404.1	STAS domain-containing protein	-
K4L72_RS02460 (512168)	512168..512533	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
K4L72_RS02465 (512536)	512536..512937	+	402	WP_003156178.1	anti-sigma regulatory factor	-
K4L72_RS02470 (512949)	512949..513956	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
K4L72_RS02475 (514020)	514020..514349	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
K4L72_RS02480 (514346)	514346..514828	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
K4L72_RS02485 (514794)	514794..515582	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
K4L72_RS02490 (515582)	515582..516184	+	603	WP_003156170.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T212765 WP_003156187.1 NZ_CP081304:510874-511224 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T212765 NZ_CP107352:2925504-2925872 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT212765 WP_003156188.1 NZ_CP081304:510587-510868 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT212765 NZ_CP107352:2925286-2925507 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5