Detailed information of TA system TA212764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2520557..2521122
• Replicon: chromosome
• Accession: NZ_CP081301
• Organism: Paenibacillus sp. PSB04

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K2F33_RS11795
• Protein ID: WP_263881767.1
• Coordinates: 2520820..2521122 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K2F33_RS11790
• Protein ID: WP_263881766.1
• Coordinates: 2520557..2520787 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2F33_RS11760 (K2F33_11735)	2516425..2517402	+	978	WP_244879440.1	AraC family transcriptional regulator	-
K2F33_RS11765 (K2F33_11740)	2517443..2517613	+	171	WP_263881762.1	hypothetical protein	-
K2F33_RS11770 (K2F33_11745)	2517697..2517999	+	303	WP_212985359.1	hypothetical protein	-
K2F33_RS11775 (K2F33_11750)	2518227..2518754	+	528	WP_212985358.1	GNAT family N-acetyltransferase	-
K2F33_RS11780 (K2F33_11755)	2518820..2519365	+	546	WP_263881764.1	HAD-IIIA family hydrolase	-
K2F33_RS11785 (K2F33_11760)	2519528..2520373	-	846	WP_263881765.1	alpha/beta hydrolase	-
K2F33_RS11790 (K2F33_11765)	2520557..2520787	+	231	WP_263881766.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
K2F33_RS11795 (K2F33_11770)	2520820..2521122	+	303	WP_263881767.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K2F33_RS11800 (K2F33_11775)	2521420..2521731	+	312	WP_263881768.1	rhodanese-like domain-containing protein	-
K2F33_RS11805 (K2F33_11780)	2521840..2522238	+	399	WP_263881769.1	rhodanese-like domain-containing protein	-
K2F33_RS11810 (K2F33_11785)	2522303..2522686	+	384	WP_089548826.1	DUF302 domain-containing protein	-
K2F33_RS11815 (K2F33_11790)	2522903..2523985	+	1083	WP_263881770.1	Gfo/Idh/MocA family oxidoreductase	-
K2F33_RS11820 (K2F33_11795)	2523982..2524827	+	846	WP_263881771.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-99)

Antitoxin

• SpoVT-AbrB domain (5-47)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11124.08 Da
• Isoelectric Point: 8.2702

>T212764 WP_263881767.1 NZ_CP081301:2520820-2521122 [Paenibacillus sp. PSB04]
VWLQFNSQAGHERAGKLPAIVISPAAYNEKVGLSLFCPITSKRKGYPFEVMIPQDLPIEGVILADQVKCLDWQSRNAVFI
CKVPQTTLSEVISKLNLLIQ

Nucleotide

• Length: 303 bp

>T212764 NZ_CP107352:c2514171-2513788 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8304.59 Da
• Isoelectric Point: 5.6664

>AT212764 WP_263881766.1 NZ_CP081301:2520557-2520787 [Paenibacillus sp. PSB04]
MFQVQKWGDSLGIRIPKSLALKVGLEEGSKVGLDVENGHLIIKLKPSSLEALLISVTPENIHKEISTGDPEGREAW

Nucleotide

• Length: 231 bp

>AT212764 NZ_CP107352:c2514413-2514171 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA