Detailed information of TA system TA212763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 384570..385196
• Replicon: chromosome
• Accession: NZ_CP081301
• Organism: Paenibacillus sp. PSB04

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: K2F33_RS01995
• Protein ID: WP_076174838.1
• Coordinates: 384570..384920 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: K2F33_RS02000
• Protein ID: WP_172679225.1
• Coordinates: 384924..385196 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2F33_RS01990 (K2F33_01980)	382171..384360	-	2190	WP_263880884.1	alpha-galactosidase	-
K2F33_RS01995 (K2F33_01985)	384570..384920	-	351	WP_076174838.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K2F33_RS02000 (K2F33_01990)	384924..385196	-	273	WP_172679225.1	ribbon-helix-helix protein, CopG family	Antitoxin
K2F33_RS02005 (K2F33_01995)	385436..386620	-	1185	WP_076174841.1	alanine racemase	-
K2F33_RS02010 (K2F33_02000)	386927..388033	-	1107	WP_076174843.1	outer membrane lipoprotein-sorting protein	-
K2F33_RS02015 (K2F33_02005)	388168..388776	-	609	WP_076174846.1	polysaccharide deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12880.84 Da
• Isoelectric Point: 5.1284

>T212763 WP_076174838.1 NZ_CP081301:c384920-384570 [Paenibacillus sp. PSB04]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAVSHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKKVDDSLQISLSLIDF

Nucleotide

• Length: 351 bp

>T212763 NZ_CP107352:c1951021-1950488 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10391.01 Da
• Isoelectric Point: 7.2027

>AT212763 WP_172679225.1 NZ_CP081301:c385196-384924 [Paenibacillus sp. PSB04]
MQNTKRIMISLPDHLLQEVDGIVAMENSNRSELIRQAMKLYLTERKKRYIRESMQRGYMEMAKINLTMASEAFHAEEDAD
STLGRLVSGV

Nucleotide

• Length: 273 bp

>AT212763 NZ_CP107352:c1951298-1951032 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA