Detailed information of TA system TA212748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 6908262..6908848
• Replicon: chromosome
• Accession: NZ_CP081202
• Organism: Pseudomonas aeruginosa strain P9W

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: G8CP73
• Locus tag: K3176_RS33055
• Protein ID: WP_003120987.1
• Coordinates: 6908262..6908561 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: K3176_RS33060
• Protein ID: WP_003448662.1
• Coordinates: 6908558..6908848 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3176_RS37950	6903264..6903908	-	645	WP_260331221.1	type IV pilus biogenesis protein PilP	-
K3176_RS33040 (K3176_33040)	6903898..6905223	-	1326	WP_003099758.1	type 4b pilus protein PilO2	-
K3176_RS33045 (K3176_33045)	6905227..6906936	-	1710	WP_003099757.1	PilN family type IVB pilus formation outer membrane protein	-
K3176_RS33050 (K3176_33050)	6906936..6908060	-	1125	WP_012076859.1	TcpQ domain-containing protein	-
K3176_RS33055 (K3176_33055)	6908262..6908561	+	300	WP_003120987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K3176_RS33060 (K3176_33060)	6908558..6908848	+	291	WP_003448662.1	putative addiction module antidote protein	Antitoxin
K3176_RS33065 (K3176_33065)	6908919..6909263	-	345	WP_023082045.1	hypothetical protein	-
K3176_RS33070 (K3176_33070)	6909402..6911378	-	1977	WP_031691363.1	DEAD/DEAH box helicase	-
K3176_RS33075 (K3176_33075)	6911375..6913264	-	1890	WP_023101908.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	6856171..6936729	80558

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11133.88 Da
• Isoelectric Point: 10.4495

>T212748 WP_003120987.1 NZ_CP081202:6908262-6908561 [Pseudomonas aeruginosa]
MVEVKQTATFMAWESKLKDRRAKAVIAARIFRLANGLPGDVSPVGQGVSELRIHYGPGYRVYFQQRGTEIVILLCGGDKS
SQARDIEMAKRLANEWRPQ

Nucleotide

• Length: 300 bp

>T212748 NZ_CP107345:4185338-4185556 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10394.91 Da
• Isoelectric Point: 4.7527

>AT212748 WP_003448662.1 NZ_CP081202:6908558-6908848 [Pseudomonas aeruginosa]
MTEQIYDYDPAQALDSPEAMALFIADALDTGDTAYIAKAMGVVARAKGMTELAKETGLAREQLYKSFSERGNPTLKTMLA
VMRALGVDLTARPHAQ

Nucleotide

• Length: 291 bp

>AT212748 NZ_CP107345:4184937-4185311 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: G8CP73