Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 480394..480983 Replicon chromosome
Accession NC_007492
Organism Pseudomonas fluorescens Pf0-1

Toxin (Protein)


Gene name PumA Uniprot ID Q3KJ93
Locus tag PFL01_RS02115 Protein ID WP_011332088.1
Coordinates 480394..480699 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q3KJ92
Locus tag PFL01_RS02120 Protein ID WP_011332089.1
Coordinates 480696..480983 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PFL01_RS02095 476414..477481 + 1068 WP_007968321.1 uroporphyrinogen decarboxylase -
PFL01_RS02100 477548..478822 - 1275 WP_011332085.1 MFS transporter -
PFL01_RS02105 478941..479837 + 897 WP_011332086.1 LysR family transcriptional regulator -
PFL01_RS02110 479838..480275 - 438 WP_011332087.1 hypothetical protein -
PFL01_RS02115 480394..480699 + 306 WP_011332088.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PFL01_RS02120 480696..480983 + 288 WP_011332089.1 putative addiction module antidote protein Antitoxin
PFL01_RS02125 481032..481472 - 441 WP_011332090.1 hypothetical protein -
PFL01_RS02130 481493..482728 - 1236 WP_011332091.1 beta-ketoacyl-ACP synthase -
PFL01_RS02135 482728..483456 - 729 WP_011332092.1 3-ketoacyl-ACP reductase FabG2 -
PFL01_RS02140 483449..483913 - 465 WP_011332093.1 hotdog family protein -
PFL01_RS02145 483910..485106 - 1197 WP_011332094.1 beta-ketoacyl-[acyl-carrier-protein] synthase family protein -
PFL01_RS02150 485103..485585 - 483 WP_011332095.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-96)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-91)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11541.14 Da        Isoelectric Point: 8.9858

>T21274 WP_011332088.1 NC_007492:480394-480699 [Pseudomonas fluorescens Pf0-1]
MTEILRSSTFSSWLVKLADSRARMRIQVRIDRMAEGNFGDVKAIDEGISEARIDYGPGYRVYFMQQGRQLVILLCGGDKS
SQSRDIKQARLIAESWQEQNP

Download         Length: 306 bp

>T21274 NC_007492:480394-480699 [Pseudomonas fluorescens Pf0-1]
ATGACTGAGATACTTCGAAGCTCCACATTCTCGAGCTGGCTTGTGAAGCTGGCTGATAGTCGCGCCAGGATGCGTATTCA
GGTGCGAATTGATCGTATGGCTGAGGGAAACTTCGGTGATGTCAAAGCCATTGACGAAGGGATCAGCGAAGCCCGAATTG
ACTATGGCCCGGGATATCGGGTCTATTTCATGCAGCAAGGTCGTCAGCTGGTCATCCTCCTTTGTGGTGGAGACAAGAGC
AGTCAATCTCGAGACATCAAGCAAGCCAGGCTCATCGCGGAATCCTGGCAGGAGCAAAACCCATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10446.86 Da        Isoelectric Point: 4.6412

>AT21274 WP_011332089.1 NC_007492:480696-480983 [Pseudomonas fluorescens Pf0-1]
MTEQFTRWDSAEYLKTEEDMANYLDACMEEAGDDPAFIAKALGTIARARGMTQVARDAGLSRESLYRALSGEVNPEFGTI
LKVMKALGLKLHAST

Download         Length: 288 bp

>AT21274 NC_007492:480696-480983 [Pseudomonas fluorescens Pf0-1]
ATGACCGAACAATTCACTCGTTGGGACTCAGCCGAGTACCTCAAGACAGAAGAGGACATGGCCAACTATCTGGATGCCTG
CATGGAAGAGGCGGGGGATGACCCGGCTTTTATTGCCAAGGCACTTGGCACGATTGCTCGGGCGCGTGGCATGACCCAGG
TCGCGCGGGATGCGGGGCTATCAAGGGAAAGTCTTTATCGCGCACTGTCAGGCGAGGTAAATCCTGAATTTGGAACGATC
CTCAAAGTCATGAAAGCGCTGGGGCTGAAGTTGCATGCCAGCACATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q3KJ93

Antitoxin

Source ID Structure
AlphaFold DB A0A345RJ97

References