Detailed information of TA system TA021273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 351927..352568
• Replicon: chromosome
• Accession: NC_007492
• Organism: Pseudomonas fluorescens Pf0-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: Q3KJK8
• Locus tag: PFL01_RS01540
• Protein ID: WP_041475489.1
• Coordinates: 351927..352109 (+)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: PFL01_RS01545
• Protein ID: WP_041475113.1
• Coordinates: 352143..352568 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PFL01_RS01500	346932..347309	+	378	WP_007952618.1	GNAT family N-acetyltransferase	-
PFL01_RS01505	347306..348022	+	717	WP_011331997.1	AzlC family ABC transporter permease	-
PFL01_RS01510	348001..348318	+	318	WP_041475112.1	AzlD domain-containing protein	-
PFL01_RS01515	348235..349161	-	927	WP_011331998.1	LysR family transcriptional regulator	-
PFL01_RS01520	349541..349759	+	219	WP_007952610.1	4-oxalocrotonate tautomerase family protein	-
PFL01_RS01525	349756..350400	+	645	WP_011331999.1	DsbA family oxidoreductase	-
PFL01_RS01530	350434..351144	+	711	WP_011332000.1	SDR family oxidoreductase	-
PFL01_RS01535	351203..351469	-	267	WP_011332001.1	DUF3077 domain-containing protein	-
PFL01_RS01540	351927..352109	+	183	WP_041475489.1	type II toxin-antitoxin system HicA family toxin	Toxin
PFL01_RS01545	352143..352568	+	426	WP_041475113.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
PFL01_RS29000	352617..352900	+	284	Protein_299	helix-turn-helix domain-containing protein	-
PFL01_RS01555	353183..353998	-	816	WP_011332005.1	FkbM family methyltransferase	-
PFL01_RS01560	354343..355578	-	1236	WP_041475114.1	methyltransferase	-
PFL01_RS01565	355559..356248	-	690	WP_007952568.1	ABC transporter permease	-
PFL01_RS01570	356245..356940	-	696	WP_011332007.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6768.82 Da
• Isoelectric Point: 10.7304

>T21273 WP_041475489.1 NC_007492:351927-352109 [Pseudomonas fluorescens Pf0-1]
MKYSEFRRWLKAQGVEFQTGKGSHFKVSLNGKSTVFPDHGAKEMGEGLRKSIVKQLGLKD

Nucleotide

• Length: 183 bp

>T21273 NC_007492:351927-352109 [Pseudomonas fluorescens Pf0-1]
ATGAAGTACAGCGAGTTTCGGCGATGGTTGAAAGCCCAAGGCGTTGAGTTCCAAACCGGCAAGGGCAGTCACTTCAAGGT
TTCCTTGAATGGCAAATCAACTGTCTTTCCCGACCACGGAGCCAAGGAGATGGGCGAAGGGTTGAGAAAATCGATAGTCA
AACAGCTGGGCCTCAAGGATTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15486.55 Da
• Isoelectric Point: 4.3686

>AT21273 WP_041475113.1 NC_007492:352143-352568 [Pseudomonas fluorescens Pf0-1]
MFEYALEVHEEPGSVWLSCAEIPEMHAAGDTLEEALDGAIDAMETALSIYVDDRRLIPTGDAGEQESDAVLRLPALTAAK
VGLWNTLLESGVSKAELARRLGVQRPQVDRLVDFLHHSKIENVERALQQLGRRILLSVEAA

Nucleotide

• Length: 426 bp

>AT21273 NC_007492:352143-352568 [Pseudomonas fluorescens Pf0-1]
ATGTTCGAATATGCGTTGGAAGTCCACGAAGAGCCGGGCAGTGTCTGGTTGTCCTGCGCAGAAATTCCAGAGATGCACGC
CGCTGGCGACACCCTCGAGGAAGCACTCGACGGCGCTATAGATGCGATGGAAACGGCATTGTCGATTTACGTCGATGATC
GCCGGTTGATTCCGACAGGTGATGCGGGAGAGCAGGAAAGTGACGCGGTATTGCGTCTGCCTGCACTGACTGCCGCAAAG
GTCGGGTTGTGGAATACGCTTCTGGAGTCTGGGGTGAGCAAGGCTGAGCTGGCACGGCGCTTGGGTGTGCAACGACCCCA
GGTAGATCGGTTAGTCGATTTCCTGCATCACTCCAAGATTGAGAATGTTGAGCGTGCGTTGCAGCAGCTTGGACGCCGGA
TTTTGCTTTCGGTTGAGGCTGCTTAG

Structures

Toxin

• AlphaFold DB: Q3KJK8