Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2922822..2923458 Replicon chromosome
Accession NZ_CP081199
Organism Bacillus pumilus strain EB130

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag K4A85_RS15130 Protein ID WP_003214169.1
Coordinates 2923108..2923458 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag K4A85_RS15125 Protein ID WP_012008995.1
Coordinates 2922822..2923103 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K4A85_RS15105 (K4A85_14735) 2918964..2919569 - 606 WP_060595589.1 rhomboid family intramembrane serine protease -
K4A85_RS15110 (K4A85_14740) 2919664..2920029 + 366 WP_034663960.1 holo-ACP synthase -
K4A85_RS15115 (K4A85_14745) 2920190..2921206 + 1017 WP_106037124.1 outer membrane lipoprotein carrier protein LolA -
K4A85_RS15120 (K4A85_14750) 2921347..2922528 + 1182 WP_066031291.1 alanine racemase -
K4A85_RS15125 (K4A85_14755) 2922822..2923103 + 282 WP_012008995.1 hypothetical protein Antitoxin
K4A85_RS15130 (K4A85_14760) 2923108..2923458 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
K4A85_RS15135 (K4A85_14765) 2923576..2924451 + 876 WP_045210228.1 RsbT co-antagonist protein RsbRA -
K4A85_RS15140 (K4A85_14770) 2924411..2924779 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
K4A85_RS15145 (K4A85_14775) 2924782..2925183 + 402 WP_060595592.1 anti-sigma regulatory factor -
K4A85_RS15150 (K4A85_14780) 2925194..2926201 + 1008 WP_060595593.1 PP2C family protein-serine/threonine phosphatase -
K4A85_RS15155 (K4A85_14785) 2926261..2926590 + 330 WP_060595594.1 anti-sigma factor antagonist -
K4A85_RS15160 (K4A85_14790) 2926587..2927075 + 489 WP_060595595.1 anti-sigma B factor RsbW -
K4A85_RS15165 (K4A85_14795) 2927041..2927829 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
K4A85_RS15170 (K4A85_14800) 2927829..2928428 + 600 WP_034663944.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T212728 WP_003214169.1 NZ_CP081199:2923108-2923458 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T212728 NZ_CP107341:c6258-4936 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCTGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT212728 WP_012008995.1 NZ_CP081199:2922822-2923103 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT212728 NZ_CP107341:c6524-6258 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References