Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1772..2319 Replicon plasmid unnamed4
Accession NZ_CP081195
Organism Escherichia coli strain EC1722-1

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag K4F91_RS25730 Protein ID WP_001384452.1
Coordinates 2041..2319 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A723YP93
Locus tag K4F91_RS25725 Protein ID WP_021559054.1
Coordinates 1772..2041 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K4F91_RS25715 (1) 1..858 + 858 WP_000131006.1 incFII family plasmid replication initiator RepA -
K4F91_RS25720 (1559) 1559..1717 + 159 WP_072649723.1 hypothetical protein -
K4F91_RS25725 (1772) 1772..2041 + 270 WP_021559054.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
K4F91_RS25730 (2041) 2041..2319 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
K4F91_RS25735 (2445) 2445..3149 - 705 WP_001067858.1 IS6-like element IS26 family transposase -
K4F91_RS25740 (3212) 3212..3811 - 600 Protein_6 ISL3-like element ISKox3 family transposase -
K4F91_RS25745 (3913) 3913..4152 + 240 WP_001549892.1 hypothetical protein -
K4F91_RS25750 (4155) 4155..4511 + 357 WP_004199403.1 hypothetical protein -
K4F91_RS25755 (4545) 4545..5249 - 705 WP_001067834.1 IS6-like element IS26 family transposase -
K4F91_RS25760 (5308) 5308..5442 + 135 WP_023408307.1 divalent cation tolerance protein CutA -
K4F91_RS26885 (5447) 5447..5821 + 375 Protein_11 protein-disulfide reductase DsbD family protein -
K4F91_RS26890 (5921) 5921..6478 + 558 Protein_12 twin-arginine translocation (TAT) pathway signal sequence domain protein -
K4F91_RS25770 (6489) 6489..7127 - 639 WP_004201168.1 phosphoribosylanthranilate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaNDM-1 / qnrS1 - 1..84890 84890


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T212726 WP_001384452.1 NZ_CP081195:2041-2319 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T212726 NZ_CP107339:c4371-4057 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10222.60 Da        Isoelectric Point: 8.8735

>AT212726 WP_021559054.1 NZ_CP081195:1772-2041 [Escherichia coli]
MAQINMSLRIDAELKDAFMAAAKSMDRNASQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT212726 NZ_CP107339:c4637-4359 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A723YP93

References