Detailed information of TA system TA212695

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 284289..285089
• Replicon: chromosome
• Accession: NZ_CP081192
• Organism: Escherichia coli strain EC1722-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4NNI0
• Locus tag: K4F91_RS01315
• Protein ID: WP_000342449.1
• Coordinates: 284562..285089 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: K4F91_RS01310
• Protein ID: WP_001277108.1
• Coordinates: 284289..284555 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K4F91_RS01290 (279946)	279946..280614	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
K4F91_RS01295 (280607)	280607..281665	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
K4F91_RS01300 (281910)	281910..282764	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
K4F91_RS01305 (283036)	283036..284139	+	1104	WP_001350438.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
K4F91_RS01310 (284289)	284289..284555	+	267	WP_001277108.1	type II toxin-antitoxin system antitoxin AtaR	Antitoxin
K4F91_RS01315 (284562)	284562..285089	+	528	WP_000342449.1	type II toxin-antitoxin system toxin acetyltransferase AtaT	Toxin
K4F91_RS01320 (285086)	285086..285469	-	384	WP_000778781.1	aspartate 1-decarboxylase autocleavage activator PanM	-
K4F91_RS01325 (285893)	285893..287002	+	1110	WP_000827696.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
K4F91_RS01330 (287050)	287050..287976	+	927	WP_000003004.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
K4F91_RS01335 (287973)	287973..289250	+	1278	WP_000803799.1	branched chain amino acid ABC transporter permease LivM	-
K4F91_RS01340 (289247)	289247..290014	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19691.70 Da
• Isoelectric Point: 7.7457

>T212695 WP_000342449.1 NZ_CP081192:284562-285089 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T212695 NZ_CP107327:882446-882853 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT212695 WP_001277108.1 NZ_CP081192:284289-284555 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT212695 NZ_CP107327:882199-882465 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A829CLZ4

Antitoxin

• AlphaFold DB: A0A829CN24