Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 262998..263584 Replicon chromosome
Accession NZ_CP081192
Organism Escherichia coli strain EC1722-1

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag K4F91_RS01190 Protein ID WP_042014162.1
Coordinates 262998..263228 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag K4F91_RS01195 Protein ID WP_000593555.1
Coordinates 263225..263584 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K4F91_RS01180 (259035) 259035..261770 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
K4F91_RS01185 (261770) 261770..262894 + 1125 WP_001314210.1 ABC transporter permease -
K4F91_RS01190 (262998) 262998..263228 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
K4F91_RS01195 (263225) 263225..263584 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
K4F91_RS01200 (263704) 263704..264105 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
K4F91_RS01205 (264111) 264111..264917 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
K4F91_RS01210 (264914) 264914..265678 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
K4F91_RS01215 (265678) 265678..266511 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
K4F91_RS01220 (266508) 266508..267452 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T212694 WP_042014162.1 NZ_CP081192:262998-263228 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T212694 NZ_CP107327:744113-744598 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT212694 WP_000593555.1 NZ_CP081192:263225-263584 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT212694 NZ_CP107327:743823-744116 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References