Detailed information of TA system TA021268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 485289..485884
• Replicon: chromosome
• Accession: NC_007484
• Organism: Nitrosococcus oceani ATCC 19707

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: Q3JDX2
• Locus tag: NOC_RS02510
• Protein ID: WP_002813764.1
• Coordinates: 485588..485884 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: NOC_RS02505
• Protein ID: WP_011330347.1
• Coordinates: 485289..485585 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOC_RS02490	480607..481899	-	1293	WP_197538940.1	restriction endonuclease subunit S	-
NOC_RS02495	481904..482395	-	492	WP_002813653.1	hypothetical protein	-
NOC_RS02500	482374..484992	-	2619	WP_002813747.1	type I restriction endonuclease subunit R	-
NOC_RS02505	485289..485585	-	297	WP_011330347.1	putative addiction module antidote protein	Antitoxin
NOC_RS02510	485588..485884	-	297	WP_002813764.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NOC_RS02515	486289..486705	-	417	WP_011330348.1	GFA family protein	-
NOC_RS02520	486789..487001	-	213	WP_002812593.1	type II toxin-antitoxin system HicB family antitoxin	-
NOC_RS16625	487012..487200	-	189	Protein_479	type II toxin-antitoxin system HicA family toxin	-
NOC_RS17240	488029..488202	+	174	WP_155814705.1	hypothetical protein	-
NOC_RS02525	488237..489145	-	909	WP_011330349.1	DUF2232 domain-containing protein	-
NOC_RS17790	489318..489581	-	264	WP_036497906.1	helix-turn-helix domain-containing protein	-
NOC_RS02535	489616..489768	+	153	WP_002813220.1	ribbon-helix-helix protein, CopG family	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (5-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11201.02 Da
• Isoelectric Point: 10.0354

>T21268 WP_002813764.1 NC_007484:c485884-485588 [Nitrosococcus oceani ATCC 19707]
MIEVVKSETFNCWLKKLRDRRAAVRISARIDRLAFGNPGNVQPIGEGLSEMRIDYGPGYRVYYMQQGKVLVLILCGGDKR
TQQEDIAKAKRIAEEWKG

Nucleotide

• Length: 297 bp

>T21268 NC_007484:c485884-485588 [Nitrosococcus oceani ATCC 19707]
ATGATCGAAGTCGTCAAATCCGAGACTTTTAACTGTTGGCTCAAAAAACTGCGGGATCGTCGAGCAGCTGTCCGTATCAG
CGCAAGAATTGATCGGCTGGCTTTCGGCAACCCTGGTAATGTTCAGCCTATAGGCGAAGGGTTGAGTGAAATGCGGATCG
ATTATGGTCCCGGATACCGGGTGTATTACATGCAGCAAGGTAAGGTTTTGGTTCTCATATTATGCGGTGGTGACAAACGT
ACACAGCAAGAAGATATTGCCAAGGCCAAACGCATAGCTGAAGAATGGAAGGGTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10687.03 Da
• Isoelectric Point: 4.7914

>AT21268 WP_011330347.1 NC_007484:c485585-485289 [Nitrosococcus oceani ATCC 19707]
MAEKFSHYDSADYLETEEDIRLYLEACQDEGGDDPAFIVHTLGVVARARNMSQLARDSGLTRAGLYKALSPEGNPSFATV
QKVAKGLGLKLTIQPISQ

Nucleotide

• Length: 297 bp

>AT21268 NC_007484:c485585-485289 [Nitrosococcus oceani ATCC 19707]
ATGGCCGAAAAATTCAGTCATTATGACAGTGCTGACTATCTTGAAACGGAAGAAGATATCCGTCTTTACCTAGAAGCCTG
CCAAGATGAAGGTGGTGATGATCCAGCATTCATCGTTCATACTCTCGGGGTTGTTGCTCGTGCTCGCAATATGAGTCAAC
TAGCCAGAGATTCAGGTCTAACCCGTGCAGGCTTATATAAGGCACTTTCCCCGGAAGGTAATCCCAGCTTTGCTACTGTC
CAAAAGGTAGCCAAGGGGCTCGGGCTAAAGCTTACTATCCAACCAATTTCACAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E2Z4B3