Detailed information of TA system TA212644

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-yefM/YoeB-YefM
• Location: 5731513..5732018
• Replicon: chromosome
• Accession: NZ_CP081178
• Organism: Pseudomonas mandelii strain KGI_MA19

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K3369_RS25885
• Protein ID: WP_077749362.1
• Coordinates: 5731764..5732018 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: -
• Locus tag: K3369_RS25880
• Protein ID: WP_010458023.1
• Coordinates: 5731513..5731767 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3369_RS25850 (K3369_25850)	5726819..5727610	+	792	WP_221107312.1	VacJ family lipoprotein	-
K3369_RS25855 (K3369_25855)	5727600..5727863	+	264	WP_221107314.1	hypothetical protein	-
K3369_RS25860 (K3369_25860)	5727969..5728331	+	363	WP_010458015.1	hypothetical protein	-
K3369_RS25865 (K3369_25865)	5728452..5730368	+	1917	WP_221107316.1	alkaline phosphatase D family protein	-
K3369_RS25870 (K3369_25870)	5730383..5730892	+	510	WP_221107318.1	adenylate kinase	-
K3369_RS25875 (K3369_25875)	5730905..5731360	+	456	WP_221107319.1	VOC family protein	-
K3369_RS25880 (K3369_25880)	5731513..5731767	+	255	WP_010458023.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
K3369_RS25885 (K3369_25885)	5731764..5732018	+	255	WP_077749362.1	Txe/YoeB family addiction module toxin	Toxin
K3369_RS25890 (K3369_25890)	5732027..5733202	-	1176	WP_221107320.1	aspartate aminotransferase family protein	-
K3369_RS25895 (K3369_25895)	5733305..5734150	+	846	WP_221107321.1	LysR family transcriptional regulator	-
K3369_RS25900 (K3369_25900)	5734282..5735295	+	1014	WP_221107327.1	sensor domain-containing diguanylate cyclase	-
K3369_RS25905 (K3369_25905)	5735292..5735696	+	405	WP_221107328.1	GNAT family N-acetyltransferase	-
K3369_RS25910 (K3369_25910)	5735709..5736416	-	708	WP_077749367.1	peptide-methionine (S)-S-oxide reductase MsrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10052.44 Da
• Isoelectric Point: 6.2190

>T212644 WP_077749362.1 NZ_CP081178:5731764-5732018 [Pseudomonas mandelii]
MNIEFTPEGWDDYLWFQQNDKAGLKRINLLITAIQREPFDGLGKPEPLKHNLSGFWSRRITAEHRLVYEVDEGEIRVVMC
RYHY

Nucleotide

• Length: 255 bp

>T212644 NZ_CP107308:746778-747263 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGCCCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCCCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9518.87 Da
• Isoelectric Point: 7.0118

>AT212644 WP_010458023.1 NZ_CP081178:5731513-5731767 [Pseudomonas mandelii]
MQTINYTTARAHLAETMDRVNEDRAPLLVTRQKGEPVVMMSLAEYNALEETAYLLRSPANAERLIKSISNLRAGKTQSRQ
LIEE

Nucleotide

• Length: 255 bp

>AT212644 NZ_CP107308:746470-746781 [Klebsiella pneumoniae]
CTGAAAACACAACCCGCTACGGGGGAGACTTACCCGAAATCTGATTTATTCAACAAAGCCAATCTGCGCATTAAACCTGC
CGAACGCGATCTTATTGATCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTG
CCGCTGAGGAAGCGCTGATTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGAT
CAGGCTCCTGCACCTAATGCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA