Detailed information of TA system TA212642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4259776..4260380
• Replicon: chromosome
• Accession: NZ_CP081178
• Organism: Pseudomonas mandelii strain KGI_MA19

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: K3369_RS19185
• Protein ID: WP_145308185.1
• Coordinates: 4259776..4260069 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A024EJX4
• Locus tag: K3369_RS19190
• Protein ID: WP_010455651.1
• Coordinates: 4260072..4260380 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3369_RS19165 (K3369_19165)	4254999..4255688	+	690	WP_077749048.1	N-acetyltransferase	-
K3369_RS19170 (K3369_19170)	4255693..4256910	+	1218	WP_010455642.1	GNAT family N-acetyltransferase	-
K3369_RS19175 (K3369_19175)	4256882..4257673	+	792	WP_083376382.1	ChbG/HpnK family deacetylase	-
K3369_RS19180 (K3369_19180)	4257789..4258481	+	693	WP_077749045.1	N-acetyltransferase	-
K3369_RS19185 (K3369_19185)	4259776..4260069	+	294	WP_145308185.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K3369_RS19190 (K3369_19190)	4260072..4260380	+	309	WP_010455651.1	putative addiction module antidote protein	Antitoxin
K3369_RS19195 (K3369_19195)	4260394..4261206	-	813	WP_221106443.1	class I SAM-dependent methyltransferase	-
K3369_RS19200 (K3369_19200)	4261386..4261772	+	387	WP_010455655.1	hypothetical protein	-
K3369_RS19205 (K3369_19205)	4261776..4263203	-	1428	WP_077749041.1	sigma-54 dependent transcriptional regulator	-
K3369_RS19210 (K3369_19210)	4263172..4264665	-	1494	WP_221106444.1	HAMP domain-containing sensor histidine kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10632.36 Da
• Isoelectric Point: 10.8628

>T212642 WP_145308185.1 NZ_CP081178:4259776-4260069 [Pseudomonas mandelii]
MNYIVQQTMIFVAWHASVRDLRAKIAIARRIDRASAGNLGDIKPVGDGVSEMRVDVGGGYRVYFTLRNSVVIVLLAGGDK
SSQTADIRRAKKLAKEV

Nucleotide

• Length: 294 bp

>T212642 NZ_CP107308:330146-330514 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11043.61 Da
• Isoelectric Point: 5.0482

>AT212642 WP_010455651.1 NZ_CP081178:4260072-4260380 [Pseudomonas mandelii]
MSQTLTTFDMAALLDSDEAISEYLSQVLAEGDNEEFLRAIGYVVKARGMTQIAKDSGMGRESLYKAFAPGAKPRFDTVLK
VIHALGIDLCAQPGHSINHSVL

Nucleotide

• Length: 309 bp

>AT212642 NZ_CP107308:329928-330149 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A024EJX4