Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 2252416..2253452 Replicon chromosome
Accession NZ_CP081106
Organism Ruegeria sp. B32

Toxin (Protein)


Gene name PP_4152 Uniprot ID -
Locus tag K3752_RS11445 Protein ID WP_260063294.1
Coordinates 2252416..2252991 (-) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID -
Locus tag K3752_RS11450 Protein ID WP_260063295.1
Coordinates 2252988..2253452 (-) Length 155 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3752_RS11425 (K3752_11380) 2247649..2250615 - 2967 WP_260063291.1 ATP-dependent DNA helicase -
K3752_RS11430 (K3752_11385) 2250767..2250964 - 198 WP_054463755.1 hypothetical protein -
K3752_RS11435 (K3752_11390) 2251263..2251517 - 255 WP_260063292.1 helix-turn-helix transcriptional regulator -
K3752_RS11440 (K3752_11395) 2251765..2252361 + 597 WP_260063293.1 DUF6088 family protein -
K3752_RS11445 (K3752_11400) 2252416..2252991 - 576 WP_260063294.1 PIN domain-containing protein Toxin
K3752_RS11450 (K3752_11405) 2252988..2253452 - 465 WP_260063295.1 helix-turn-helix domain-containing protein Antitoxin
K3752_RS11455 (K3752_11410) 2253641..2254504 - 864 WP_260063296.1 hypothetical protein -
K3752_RS11460 (K3752_11415) 2255021..2255977 - 957 WP_260063297.1 NAD(P)-dependent oxidoreductase -
K3752_RS11465 (K3752_11420) 2256086..2256961 + 876 WP_260063298.1 LysR substrate-binding domain-containing protein -
K3752_RS11470 (K3752_11425) 2257321..2257704 + 384 WP_093962591.1 DUF1636 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 2210795..2254802 44007


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(11-115)

Antitoxin

SinI-like, DNA-binding domain

(85-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21014.07 Da        Isoelectric Point: 4.4620

>T212632 WP_260063294.1 NZ_CP081106:c2252991-2252416 [Ruegeria sp. B32]
MSDYADRFTALIDANVLACAMRRNIILSLAEAGFFRPRWSDRIMDETAGAIAKITKGGADTNKQRSAMERAFPEACVTGY
AGLEAGLQLPDPDDRHVLAAAITTSAQVIVTENLKDFPSEALVPFDIDAISADEFISDAIELDPLAAVVALRKMRERFKN
PDMDAEALLLRIKARALFQTAEILHEYVELL

Download         Length: 576 bp

>T212632 NZ_CP107303:2808009-2808392 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 155 a.a.        Molecular weight: 17157.62 Da        Isoelectric Point: 4.5611

>AT212632 WP_260063295.1 NZ_CP081106:c2253452-2252988 [Ruegeria sp. B32]
MMMQANEAFAERLPDQVEIENADSLRRILASQVNGDEAVELSLAFGEKQIEPVTLAPALAASLMELLRLVSSGRGFRMIP
VDAELTTQQAADLLNVSRPHLVKLLEDGEIAFFKTGRHRRVKAADLFEYKKKRDTDRSDALSDLAAMDMENDLL

Download         Length: 465 bp

>AT212632 NZ_CP107303:2807767-2808009 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References