212631

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 63212..63789 Replicon plasmid unnamed1
Accession NZ_CP081071
Organism Leisingera caerulea strain S122

Toxin (Protein)


Gene name PumA Uniprot ID A0A1B8S200
Locus tag K3721_RS19045 Protein ID WP_065267708.1
Coordinates 63502..63789 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A1B8S1Z8
Locus tag K3721_RS19040 Protein ID WP_065267707.1
Coordinates 63212..63499 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3721_RS19005 (K3721_18950) 58716..59027 - 312 WP_259972814.1 H-NS histone family protein -
K3721_RS19010 (K3721_18960) 59496..60155 - 660 WP_259972815.1 hypothetical protein -
K3721_RS19015 (K3721_18965) 60189..60704 - 516 WP_259972816.1 hypothetical protein -
K3721_RS19020 (K3721_18970) 61045..61287 + 243 WP_259972706.1 helix-turn-helix transcriptional regulator -
K3721_RS19025 (K3721_18975) 61288..61521 + 234 WP_259972707.1 hypothetical protein -
K3721_RS19030 (K3721_18980) 61745..62221 + 477 WP_259972708.1 hypothetical protein -
K3721_RS19035 (K3721_18985) 62978..63178 + 201 WP_259972709.1 hypothetical protein -
K3721_RS19040 (K3721_18990) 63212..63499 - 288 WP_065267707.1 putative addiction module antidote protein Antitoxin
K3721_RS19045 (K3721_18995) 63502..63789 - 288 WP_065267708.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K3721_RS19050 (K3721_19000) 63859..64152 - 294 WP_259972710.1 hypothetical protein -
K3721_RS19055 (K3721_19005) 64164..64634 - 471 WP_259972711.1 hypothetical protein -
K3721_RS19060 (K3721_19010) 64631..64819 - 189 WP_259972712.1 hypothetical protein -
K3721_RS19065 (K3721_19015) 64816..65043 - 228 WP_259972713.1 hypothetical protein -
K3721_RS19070 (K3721_19020) 65482..65673 - 192 WP_222506807.1 hypothetical protein -
K3721_RS19075 (K3721_19025) 66468..67208 + 741 WP_259972714.1 hypothetical protein -
K3721_RS19080 (K3721_19030) 67177..67479 + 303 WP_141889271.1 hypothetical protein -
K3721_RS19085 (K3721_19035) 67721..68071 + 351 WP_259972715.1 hypothetical protein -
K3721_RS19090 (K3721_19040) 68244..68462 - 219 WP_259972716.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..121429 121429


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-94)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10894.70 Da        Isoelectric Point: 10.2193

>T212631 WP_065267708.1 NZ_CP081071:c63789-63502 [Leisingera caerulea]
MIEVQQTEEFSRWMRKLKDRRAVARIAMRIERLEAGGLGDVKFFDGIGELRIDYGPGYRVYFAKRGDILIILLCGGDKSS
QRRDIARAVSMAKEV

Download         Length: 288 bp

>T212631 NZ_CP107303:c2401122-2400754 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10088.61 Da        Isoelectric Point: 4.7863

>AT212631 WP_065267707.1 NZ_CP081071:c63499-63212 [Leisingera caerulea]
MPIKTTRFDAAEYLDTIEAQAEYLAAAFETGDEAYIKNAMATVARARGMTEIAKAADVTRQALYKGLSEAGDPKLSTVLG
VMKALGMKVTVEAGV

Download         Length: 288 bp

>AT212631 NZ_CP107303:c2401340-2401119 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8S200

Antitoxin

Source ID Structure
AlphaFold DB A0A1B8S1Z8

References