Detailed information of TA system TA212630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 25408..25939
• Replicon: plasmid unnamed2
• Accession: NZ_CP081060
• Organism: Leisingera aquaemixtae strain M602

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: K3719_RS19430
• Protein ID: WP_259947435.1
• Coordinates: 25637..25939 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: K3719_RS19425
• Protein ID: WP_259947433.1
• Coordinates: 25408..25644 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3719_RS19420 (K3719_19350)	21406..25263	+	3858	WP_259947470.1	PEP/pyruvate-binding domain-containing protein	-
K3719_RS19425 (K3719_19355)	25408..25644	+	237	WP_259947433.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
K3719_RS19430 (K3719_19360)	25637..25939	+	303	WP_259947435.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K3719_RS19435 (K3719_19365)	25989..26900	+	912	WP_259947437.1	MurR/RpiR family transcriptional regulator	-
K3719_RS19440 (K3719_19370)	26910..27344	-	435	WP_259947439.1	GFA family protein	-
K3719_RS19445 (K3719_19375)	27411..28685	-	1275	WP_259947441.1	nucleotide sugar dehydrogenase	-
K3719_RS19450 (K3719_19380)	28791..29474	-	684	WP_141892205.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	hsiB1/vipA / hsiC1/vipB / clpV1	1..108687	108687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (7-93)

Antitoxin

• Antitoxin ParD (1-75)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11490.16 Da
• Isoelectric Point: 6.7263

>T212630 WP_259947435.1 NZ_CP081060:25637-25939 [Leisingera aquaemixtae]
MAEPRWRIRPAARADLAAIWRYGRDTWGAAQADAYADSLFALFDVLADFPEMARERAEFTPPVRIHPSGAHLVLYRLEQG
RPEILRILHARQDLMAFLSE

Nucleotide

• Length: 303 bp

>T212630 NZ_CP107303:c2375151-2374804 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8488.46 Da
• Isoelectric Point: 4.6721

>AT212630 WP_259947433.1 NZ_CP081060:25408-25644 [Leisingera aquaemixtae]
MATMNVSLPDQMKDWVEEQARTGTYANSSDYVRDLIRRDQARTAAIAELQTAIDAGIASGPAEPLSAESFKAAMRRNG

Nucleotide

• Length: 237 bp

>AT212630 NZ_CP107303:c2374804-2374505 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA