Detailed information of TA system TA021263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 33725..34265
• Replicon: plasmid A
• Accession: NC_007483
• Organism: Nitrosococcus oceani ATCC 19707

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: D8KCE2
• Locus tag: NOC_RS00185
• Protein ID: WP_004164066.1
• Coordinates: 33725..34045 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: D8KCE1
• Locus tag: NOC_RS00190
• Protein ID: WP_004164082.1
• Coordinates: 34032..34265 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NOC_RS00165	28840..30180	+	1341	WP_004164074.1	GIY-YIG nuclease family protein	-
NOC_RS00170	30330..30629	-	300	WP_004164096.1	hypothetical protein	-
NOC_RS00175	30793..31803	-	1011	WP_011330193.1	Abi family protein	-
NOC_RS00180	32615..33721	-	1107	WP_004164104.1	metal-dependent hydrolase	-
NOC_RS00185	33725..34045	-	321	WP_004164066.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
NOC_RS00190	34032..34265	-	234	WP_004164082.1	hypothetical protein	Antitoxin
NOC_RS00195	34329..35303	-	975	WP_166485330.1	ATP-binding protein	-
NOC_RS00205	35945..36181	-	237	WP_004164080.1	hypothetical protein	-
NOC_RS00210	36262..36597	-	336	WP_011330196.1	helix-turn-helix transcriptional regulator	-
NOC_RS00215	37051..37818	-	768	WP_004164047.1	hypothetical protein	-
NOC_RS00220	38194..38472	-	279	WP_004164067.1	hypothetical protein	-
NOC_RS00225	38614..38844	-	231	WP_004164073.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..40420	40420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11531.59 Da
• Isoelectric Point: 11.1935

>T21263 WP_004164066.1 NC_007483:c34045-33725 [Nitrosococcus oceani ATCC 19707]
MTRGEVYWVNLNPTQGSEIRKRRPCVLVGATPINQARRTVVVVPLSTSAKPRPPLTVPIQGLAQPVVAVCDQIRAVDKSR
LLEAAGRLSKKDLDALAEGLRQVLVL

Nucleotide

• Length: 321 bp

>T21263 NC_007483:c34045-33725 [Nitrosococcus oceani ATCC 19707]
ATGACTCGGGGTGAGGTGTATTGGGTGAATCTTAATCCCACCCAAGGGTCGGAAATCCGCAAGCGCCGCCCCTGCGTCTT
GGTGGGGGCCACGCCCATCAATCAGGCCCGGCGCACGGTAGTGGTCGTGCCCTTGTCCACCTCCGCCAAGCCCAGGCCGC
CCCTGACCGTGCCTATCCAGGGCCTTGCTCAGCCCGTGGTGGCGGTCTGCGATCAGATCCGTGCGGTGGATAAGTCCCGC
TTGCTAGAGGCAGCCGGCCGTTTATCCAAAAAAGACTTGGACGCTCTGGCGGAAGGGCTGAGGCAGGTGTTGGTCCTTTA
G

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8635.85 Da
• Isoelectric Point: 4.4217

>AT21263 WP_004164082.1 NC_007483:c34265-34032 [Nitrosococcus oceani ATCC 19707]
MQKVLFSLPPDLVARMRASIPPKQRSQVIARLLAQELEKREQALYQCALEVEADDALGEEMKDWDITVGDGLNHDSG

Nucleotide

• Length: 234 bp

>AT21263 NC_007483:c34265-34032 [Nitrosococcus oceani ATCC 19707]
ATGCAAAAAGTGCTCTTTTCCTTGCCCCCTGACTTGGTGGCGCGCATGCGGGCCAGCATCCCGCCGAAACAACGCAGCCA
GGTGATCGCCCGCCTCCTGGCGCAGGAGCTTGAGAAGCGGGAGCAGGCCCTTTATCAATGCGCCCTGGAAGTGGAGGCAG
ACGATGCCCTGGGGGAAGAAATGAAAGACTGGGACATCACCGTGGGCGATGGCCTCAACCATGACTCGGGGTGA

Structures

Toxin

• AlphaFold DB: D8KCE2

Antitoxin

• AlphaFold DB: D8KCE1