Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 599029..599461 Replicon chromosome
Accession NZ_CP081019
Organism Clostridioides difficile strain CD099

Toxin (Protein)


Gene name zeta Uniprot ID R4ZD79
Locus tag K2218_RS03165 Protein ID WP_003423072.1
Coordinates 599029..599187 (-) Length 53 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag K2218_RS03170 Protein ID WP_000301765.1
Coordinates 599189..599461 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K2218_RS03130 594713..594913 - 201 WP_021405844.1 helix-turn-helix domain-containing protein -
K2218_RS03135 (K2218_03135) 595365..595613 - 249 WP_002323370.1 helix-turn-helix domain-containing protein -
K2218_RS03140 (K2218_03140) 595620..595901 - 282 Protein_518 sigma-70 family RNA polymerase sigma factor -
K2218_RS03145 (K2218_03145) 596363..597241 - 879 WP_003423074.1 ParA family protein -
K2218_RS03150 (K2218_03150) 597344..597604 - 261 Protein_520 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
K2218_RS03155 (K2218_03155) 597774..598511 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
K2218_RS03160 (K2218_03160) 598621..598977 - 357 Protein_522 AAA family ATPase -
K2218_RS03165 (K2218_03165) 599029..599187 - 159 WP_003423072.1 zeta toxin family protein Toxin
K2218_RS03170 (K2218_03170) 599189..599461 - 273 WP_000301765.1 antitoxin Antitoxin
K2218_RS03175 (K2218_03175) 599478..599693 - 216 WP_002321610.1 peptide-binding protein -
K2218_RS03180 (K2218_03180) 599880..600239 + 360 WP_003423065.1 helix-turn-helix transcriptional regulator -
K2218_RS03185 (K2218_03185) 600347..600535 - 189 WP_009269672.1 cysteine-rich KTR domain-containing protein -
K2218_RS03190 (K2218_03190) 600740..600931 - 192 WP_003423063.1 hypothetical protein -
K2218_RS03195 (K2218_03195) 600944..601588 - 645 WP_003423062.1 ABC-2 transporter permease -
K2218_RS03200 (K2218_03200) 601585..602448 - 864 WP_003423060.1 ABC transporter ATP-binding protein -
K2218_RS03205 (K2218_03205) 602449..602826 - 378 WP_003423058.1 GntR family transcriptional regulator -
K2218_RS03210 (K2218_03210) 602947..603858 - 912 WP_003423057.1 conjugal transfer protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) clpP 593249..635492 42243


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(26-50)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5776.53 Da        Isoelectric Point: 10.3088

>T212601 WP_003423072.1 NZ_CP081019:c599187-599029 [Clostridioides difficile]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRRR

Download         Length: 159 bp

>T212601 NZ_CP107291:c5119833-5118916 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT212601 WP_000301765.1 NZ_CP081019:c599461-599189 [Clostridioides difficile]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT212601 NZ_CP107291:c5120597-5119839 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGTCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4ZD79

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References