Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 3871114..3871546 Replicon chromosome
Accession NZ_CP081017
Organism Clostridioides difficile strain SH

Toxin (Protein)


Gene name zeta Uniprot ID R4ZD79
Locus tag K2217_RS17730 Protein ID WP_003423072.1
Coordinates 3871388..3871546 (+) Length 53 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag K2217_RS17725 Protein ID WP_000301765.1
Coordinates 3871114..3871386 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K2217_RS17685 (K2217_17675) 3866717..3867628 + 912 WP_003423057.1 conjugal transfer protein -
K2217_RS17690 (K2217_17680) 3867749..3868126 + 378 WP_003423058.1 GntR family transcriptional regulator -
K2217_RS17695 (K2217_17685) 3868127..3868990 + 864 WP_003423060.1 ABC transporter ATP-binding protein -
K2217_RS17700 (K2217_17690) 3868987..3869631 + 645 WP_003423062.1 ABC-2 transporter permease -
K2217_RS17705 (K2217_17695) 3869644..3869835 + 192 WP_003423063.1 hypothetical protein -
K2217_RS17710 (K2217_17700) 3870040..3870228 + 189 WP_009269672.1 cysteine-rich KTR domain-containing protein -
K2217_RS17715 (K2217_17705) 3870336..3870695 - 360 WP_003423065.1 helix-turn-helix transcriptional regulator -
K2217_RS17720 (K2217_17710) 3870882..3871097 + 216 WP_002321610.1 peptide-binding protein -
K2217_RS17725 (K2217_17715) 3871114..3871386 + 273 WP_000301765.1 antitoxin Antitoxin
K2217_RS17730 (K2217_17720) 3871388..3871546 + 159 WP_003423072.1 zeta toxin family protein Toxin
K2217_RS17735 (K2217_17725) 3871598..3871954 + 357 Protein_3427 AAA family ATPase -
K2217_RS17740 (K2217_17730) 3872064..3872801 + 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
K2217_RS17745 (K2217_17735) 3872971..3873231 + 261 Protein_3429 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
K2217_RS17750 (K2217_17740) 3873334..3874212 + 879 WP_003423074.1 ParA family protein -
K2217_RS17755 (K2217_17745) 3874674..3874955 + 282 Protein_3431 sigma-70 family RNA polymerase sigma factor -
K2217_RS17760 (K2217_17750) 3874962..3875210 + 249 WP_002323370.1 helix-turn-helix domain-containing protein -
K2217_RS17765 3875662..3875862 + 201 WP_021405844.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element erm(B) - 3850091..3877326 27235


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(26-50)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 53 a.a.        Molecular weight: 5776.53 Da        Isoelectric Point: 10.3088

>T212600 WP_003423072.1 NZ_CP081017:3871388-3871546 [Clostridioides difficile]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRRR

Download         Length: 159 bp

>T212600 NZ_CP107291:c4963016-4962732 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT212600 WP_000301765.1 NZ_CP081017:3871114-3871386 [Clostridioides difficile]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT212600 NZ_CP107291:c4963248-4963006 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R4ZD79

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References