Detailed information of TA system TA212589

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 2977295..2977842
• Replicon: chromosome
• Accession: NZ_CP081016
• Organism: Pseudomonas sp. 2hn

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: K2O50_RS13400
• Protein ID: WP_050705773.1
• Coordinates: 2977295..2977570 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K2O50_RS13405
• Protein ID: WP_050705772.1
• Coordinates: 2977570..2977842 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2O50_RS13370 (K2O50_13370)	2972592..2972828	+	237	WP_028688843.1	hypothetical protein	-
K2O50_RS13375 (K2O50_13375)	2972905..2973213	+	309	WP_050705778.1	DUF3077 domain-containing protein	-
K2O50_RS13380 (K2O50_13380)	2973408..2973971	+	564	WP_050705777.1	hypothetical protein	-
K2O50_RS13385 (K2O50_13385)	2973996..2974379	+	384	WP_050705776.1	hypothetical protein	-
K2O50_RS13390 (K2O50_13390)	2974520..2975257	-	738	WP_050705775.1	hypothetical protein	-
K2O50_RS13395 (K2O50_13395)	2975538..2977145	+	1608	WP_050705774.1	methyl-accepting chemotaxis protein	-
K2O50_RS13400 (K2O50_13400)	2977295..2977570	-	276	WP_050705773.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K2O50_RS13405 (K2O50_13405)	2977570..2977842	-	273	WP_050705772.1	ribbon-helix-helix domain-containing protein	Antitoxin
K2O50_RS13410 (K2O50_13410)	2978054..2980291	-	2238	WP_050705771.1	TonB-dependent receptor	-
K2O50_RS13415 (K2O50_13415)	2980529..2981491	-	963	WP_050705770.1	siderophore ABC transporter substrate-binding protein	-
K2O50_RS13420 (K2O50_13420)	2981488..2982270	-	783	WP_050705769.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2962759..2977842	15083

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10660.28 Da
• Isoelectric Point: 8.5491

>T212589 WP_050705773.1 NZ_CP081016:c2977570-2977295 [Pseudomonas sp. 2hn]
MDLKWTSKALSDIARLYEFLAAVNKPAAVRTVQQLTGAASMLLANPRIGERLEEFEPRDVRRIQIGHYEMRYEIADSTLF
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T212589 NZ_CP107287:c30004-29756 [Escherichia coli]
ATGAGAAAAAATAAAGGCGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCCGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAAAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10153.55 Da
• Isoelectric Point: 5.6994

>AT212589 WP_050705772.1 NZ_CP081016:c2977842-2977570 [Pseudomonas sp. 2hn]
MATTIKTRSVTAHVPVQLAEKVDLLAERLERSKNWIVKQALIAWIDQEEERSRLTREALADVDANRVIDHQAVQAWADSL
GTDTPLPVPR

Nucleotide

• Length: 273 bp

>AT212589 NZ_CP107287:c29766-29539 [Escherichia coli]
ATGAAACCTGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA