Detailed information of TA system TA212586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 682880..683520
• Replicon: chromosome
• Accession: NZ_CP081016
• Organism: Pseudomonas sp. 2hn

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: K2O50_RS03255
• Protein ID: WP_050707095.1
• Coordinates: 682880..683290 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q1I4A9
• Locus tag: K2O50_RS03260
• Protein ID: WP_011535889.1
• Coordinates: 683290..683520 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2O50_RS03230 (K2O50_03230)	679875..680306	-	432	WP_220689970.1	DUF2946 domain-containing protein	-
K2O50_RS03235 (K2O50_03235)	680332..680817	-	486	WP_050707099.1	copper chaperone PCu(A)C	-
K2O50_RS03240 (K2O50_03240)	680865..681254	-	390	WP_050707098.1	DUF2946 domain-containing protein	-
K2O50_RS03245 (K2O50_03245)	681488..682342	-	855	WP_050707097.1	SPFH domain-containing protein	-
K2O50_RS03250 (K2O50_03250)	682370..682807	-	438	WP_050707096.1	NfeD family protein	-
K2O50_RS03255 (K2O50_03255)	682880..683290	-	411	WP_050707095.1	tRNA(fMet)-specific endonuclease VapC	Toxin
K2O50_RS03260 (K2O50_03260)	683290..683520	-	231	WP_011535889.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K2O50_RS03265 (K2O50_03265)	683603..684331	-	729	WP_050707094.1	cobalt-precorrin-6A reductase	-
K2O50_RS03270 (K2O50_03270)	684328..685422	-	1095	WP_050707093.1	cobalt-precorrin-5B (C(1))-methyltransferase	-
K2O50_RS03275 (K2O50_03275)	685415..686626	-	1212	WP_050707092.1	bifunctional cobalt-precorrin-7 (C(5))-methyltransferase/cobalt-precorrin-6B (C(15))-methyltransferase	-
K2O50_RS03280 (K2O50_03280)	686842..688167	+	1326	WP_050707091.1	precorrin-3B synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15260.62 Da
• Isoelectric Point: 9.4581

>T212586 WP_050707095.1 NZ_CP081016:c683290-682880 [Pseudomonas sp. 2hn]
MLRYMLDTNICIFTIKNKPQVVREAFNRYHGQLAISTVTLMELIYGAEKSAAPERNLAIVEGFAARLDVLDYDIQGAAHT
AQLRAELAKAGTPIGPYDRMIAGHARARGLTLVTNNLREFQRVPGLRVEDWVTHPR

Nucleotide

• Length: 411 bp

>T212586 NZ_CP107284:c4526705-4526532 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTTTACCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8443.36 Da
• Isoelectric Point: 4.2821

>AT212586 WP_011535889.1 NZ_CP081016:c683520-683290 [Pseudomonas sp. 2hn]
MEQGAVFKSNRSQAIRMPKALALPEDVTRVDIVAVGRSRIISPAGESWDSWFEGDDVSADFMASREQPADQEREGF

Nucleotide

• Length: 231 bp

>AT212586 NZ_CP107284:c4527029-4526733 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGCATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A2V4HYG6