Detailed information of TA system TA212584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 316365..316970
• Replicon: chromosome
• Accession: NZ_CP081016
• Organism: Pseudomonas sp. 2hn

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: K2O50_RS01505
• Protein ID: WP_050703403.1
• Coordinates: 316365..316655 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: K2O50_RS01510
• Protein ID: WP_050703404.1
• Coordinates: 316665..316970 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K2O50_RS01485 (K2O50_01485)	311715..312488	-	774	WP_050703399.1	ABC transporter ATP-binding protein	-
K2O50_RS01490 (K2O50_01490)	313095..314480	+	1386	WP_050703400.1	GABA permease	-
K2O50_RS01495 (K2O50_01495)	314555..314998	-	444	WP_050703401.1	hypothetical protein	-
K2O50_RS01500 (K2O50_01500)	315191..316201	-	1011	WP_050703402.1	polyphosphate kinase 2	-
K2O50_RS01505 (K2O50_01505)	316365..316655	+	291	WP_050703403.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K2O50_RS01510 (K2O50_01510)	316665..316970	+	306	WP_050703404.1	putative addiction module antidote protein	Antitoxin
K2O50_RS01520 (K2O50_01520)	317242..317514	-	273	WP_011536185.1	oxidative damage protection protein	-
K2O50_RS01525 (K2O50_01525)	317511..318578	-	1068	WP_050703405.1	A/G-specific adenine glycosylase	-
K2O50_RS01530 (K2O50_01530)	318575..320824	-	2250	WP_050703406.1	AsmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10946.80 Da
• Isoelectric Point: 10.7046

>T212584 WP_050703403.1 NZ_CP081016:316365-316655 [Pseudomonas sp. 2hn]
MHTLIQTDMYRKWFAALRDARAQARINVRLRRVELGVLGDCKPVGEGVFELRIDYGPGYRVYLARRGYEVIILLAGGDKA
TQARDIKSALRLARNL

Nucleotide

• Length: 291 bp

>T212584 NZ_CP107284:c4413290-4413000 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10804.43 Da
• Isoelectric Point: 4.6135

>AT212584 WP_050703404.1 NZ_CP081016:316665-316970 [Pseudomonas sp. 2hn]
MTDLKLTKWDAADHLQTEEDIVLYLEACLEENDPTLLAAALGDIARARGMAQLARDTGLTREGLYKALSADGNPSLATIM
KVMAALGVRLHAEIVTPRAAG

Nucleotide

• Length: 306 bp

>AT212584 NZ_CP107284:c4413522-4413280 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGACTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA