Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 12997..13519 Replicon plasmid p25POL-B
Accession NZ_CP081012
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag K3072_RS25020 Protein ID WP_000220560.1
Coordinates 13238..13519 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag K3072_RS25015 Protein ID WP_000121743.1
Coordinates 12997..13248 (+) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS24990 (8156) 8156..9133 + 978 WP_001269914.1 hypothetical protein -
K3072_RS24995 (9130) 9130..9486 - 357 WP_001546351.1 DNA distortion polypeptide 3 -
K3072_RS25000 (9626) 9626..10069 - 444 WP_001074379.1 hypothetical protein -
K3072_RS25005 (10073) 10073..10915 - 843 WP_000435063.1 replication initiation protein -
K3072_RS25010 (10936) 10936..11775 - 840 WP_001546350.1 replication initiation protein -
K3072_RS25015 (12997) 12997..13248 + 252 WP_000121743.1 plasmid stabilization protein Antitoxin
K3072_RS25020 (13238) 13238..13519 + 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K3072_RS25025 (13665) 13665..13988 + 324 WP_001181903.1 hypothetical protein -
K3072_RS25030 (14033) 14033..14278 + 246 WP_000356548.1 hypothetical protein -
K3072_RS25035 (14268) 14268..14483 + 216 WP_001180116.1 hypothetical protein -
K3072_RS25040 (14576) 14576..14905 + 330 WP_000866648.1 hypothetical protein -
K3072_RS25045 (14948) 14948..15127 + 180 WP_071526261.1 hypothetical protein -
K3072_RS25050 (15213) 15213..15362 + 150 WP_000003880.1 hypothetical protein -
K3072_RS25055 (15375) 15375..15647 + 273 WP_000160399.1 hypothetical protein -
K3072_RS25060 (15878) 15878..16231 + 354 Protein_23 H-NS histone family protein -
K3072_RS25065 (16286) 16286..16693 + 408 WP_001546356.1 hypothetical protein -
K3072_RS25070 (16950) 16950..17414 + 465 WP_001443073.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..24185 24185


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-86)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T212581 WP_000220560.1 NZ_CP081012:13238-13519 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T212581 NZ_CP107284:c3914915-3914517 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTTGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT212581 WP_000121743.1 NZ_CP081012:12997-13248 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT212581 NZ_CP107284:c3915211-3914918 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G3CAN5


Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References