Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4607476..4607973 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K3072_RS22205 Protein ID WP_001173343.1
Coordinates 4607476..4607649 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag K3072_RS22210 Protein ID WP_001312331.1
Coordinates 4607677..4607973 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS22195 (4604146) 4604146..4605603 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
K3072_RS22200 (4605840) 4605840..4607357 + 1518 WP_000003806.1 lysine--tRNA ligase -
K3072_RS22205 (4607476) 4607476..4607649 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K3072_RS22210 (4607677) 4607677..4607973 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K3072_RS22215 (4608202) 4608202..4608474 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
K3072_RS22220 (4608486) 4608486..4608716 - 231 WP_000371715.1 4Fe-4S mono-cluster protein YjdI -
K3072_RS22225 (4608897) 4608897..4610528 + 1632 WP_001216466.1 sensor histidine kinase -
K3072_RS22230 (4610525) 4610525..4611244 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T212578 WP_001173343.1 NZ_CP081010:c4607649-4607476 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T212578 NZ_CP107284:c2973165-2972782 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTTACGTTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGTGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCG
GGACAATTACAGAATGGTAAATAACATTACCCTGGGCAAGCATCCGGAGGAGGCGAAACAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT212578 WP_001312331.1 NZ_CP081010:c4607973-4607677 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT212578 NZ_CP107284:c2973626-2973246 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAACAAAGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCAATGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGGCATCAGCATACCGTCACGCTGTATGCGAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCTGCGACCGAAACGGAAAGTAACCCTCCGGAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References