Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4419006..4419841 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J2AEA6
Locus tag K3072_RS21275 Protein ID WP_000854759.1
Coordinates 4419006..4419383 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1NM52
Locus tag K3072_RS21280 Protein ID WP_001295723.1
Coordinates 4419473..4419841 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS21250 (4415117) 4415117..4416739 - 1623 WP_001295726.1 Alw26I/Eco31I/Esp3I family type II restriction adenine-specific DNA-methyltransferase -
K3072_RS21255 (4417530) 4417530..4417706 - 177 Protein_4183 helix-turn-helix domain-containing protein -
K3072_RS21260 (4418073) 4418073..4418222 - 150 Protein_4184 hypothetical protein -
K3072_RS21265 (4418328) 4418328..4418504 - 177 WP_000839286.1 DUF957 domain-containing protein -
K3072_RS21270 (4418521) 4418521..4419009 - 489 WP_000761690.1 DUF5983 family protein -
K3072_RS21275 (4419006) 4419006..4419383 - 378 WP_000854759.1 TA system toxin CbtA family protein Toxin
K3072_RS21280 (4419473) 4419473..4419841 - 369 WP_001295723.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
K3072_RS21285 (4420004) 4420004..4420225 - 222 WP_000692345.1 DUF987 domain-containing protein -
K3072_RS21290 (4420288) 4420288..4420764 - 477 WP_001186775.1 RadC family protein -
K3072_RS21295 (4420780) 4420780..4421253 - 474 WP_001350782.1 antirestriction protein -
K3072_RS21300 (4421347) 4421347..4421592 - 246 WP_001164966.1 hypothetical protein -
K3072_RS21305 (4421592) 4421592..4422410 - 819 WP_001234738.1 DUF932 domain-containing protein -
K3072_RS21310 (4422528) 4422528..4422723 - 196 Protein_4194 DUF905 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4407465..4434024 26559


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14144.26 Da        Isoelectric Point: 7.3249

>T212577 WP_000854759.1 NZ_CP081010:c4419383-4419006 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDLPGF
SACTHSQLINSIDILRARRATGLMIRDNYRTVNNITLGKHPEAKR

Download         Length: 378 bp

>T212577 NZ_CP107284:2763988-2764095 [Escherichia coli]
ATGACGCTCACGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAACACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13592.40 Da        Isoelectric Point: 6.6255

>AT212577 WP_001295723.1 NZ_CP081010:c4419841-4419473 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT212577 NZ_CP107284:c2763938-2763875 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTATACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2AEA6

Antitoxin

Source ID Structure
AlphaFold DB S1NM52

References