Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4336944..4337520 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name shpA Uniprot ID A0A061KXE4
Locus tag K3072_RS20880 Protein ID WP_001295743.1
Coordinates 4337233..4337520 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag K3072_RS20875 Protein ID WP_000063148.1
Coordinates 4336944..4337246 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS20860 (4333540) 4333540..4335690 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
K3072_RS20865 (4335740) 4335740..4335943 + 204 WP_000467859.1 YbdD/YjiX family protein -
K3072_RS20870 (4335954) 4335954..4336910 + 957 WP_001295745.1 GTPase -
K3072_RS20875 (4336944) 4336944..4337246 - 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
K3072_RS20880 (4337233) 4337233..4337520 - 288 WP_001295743.1 BrnT family toxin Toxin
K3072_RS20885 (4337717) 4337717..4338883 + 1167 WP_000800831.1 restriction endonuclease -
K3072_RS20890 (4338947) 4338947..4340566 + 1620 WP_001029745.1 class I SAM-dependent DNA methyltransferase -
K3072_RS20895 (4340556) 4340556..4341860 + 1305 WP_000535012.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11156.62 Da        Isoelectric Point: 7.4697

>T212576 WP_001295743.1 NZ_CP081010:c4337520-4337233 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T212576 NZ_CP107284:2763453-2763560 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT212576 WP_000063148.1 NZ_CP081010:c4337246-4336944 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT212576 NZ_CP107284:c2763400-2763339 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KXE4

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References