Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4231656..4232206 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag K3072_RS20360 Protein ID WP_001160966.1
Coordinates 4231656..4231970 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag K3072_RS20365 Protein ID WP_000125566.1
Coordinates 4231973..4232206 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS20335 (4227331) 4227331..4228617 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
K3072_RS20340 (4228617) 4228617..4229606 + 990 WP_000241229.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
K3072_RS20345 (4229603) 4229603..4230424 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
K3072_RS20350 (4230427) 4230427..4230804 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
K3072_RS20355 (4230811) 4230811..4231659 + 849 WP_000257182.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
K3072_RS20360 (4231656) 4231656..4231970 - 315 WP_001160966.1 CcdB family protein Toxin
K3072_RS20365 (4231973) 4231973..4232206 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
K3072_RS20370 (4232292) 4232292..4232771 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
K3072_RS20375 (4232963) 4232963..4234825 - 1863 WP_000377123.1 glutathione-regulated potassium-efflux system protein KefC -
K3072_RS20380 (4234818) 4234818..4235348 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
K3072_RS20385 (4235456) 4235456..4236787 - 1332 WP_001183207.1 MFS transporter -
K3072_RS20390 (4236846) 4236846..4237133 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T212573 WP_001160966.1 NZ_CP081010:c4231970-4231656 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T212573 NZ_CP107284:1054443-1054778 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTCTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAAAGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT212573 WP_000125566.1 NZ_CP081010:c4232206-4231973 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT212573 NZ_CP107284:1054195-1054443 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCAGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References