Detailed information of TA system TA212570

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3092301..3093135
• Replicon: chromosome
• Accession: NZ_CP081010
• Organism: Escherichia coli strain 25POL

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: K3072_RS15010
• Protein ID: WP_000854690.1
• Coordinates: 3092301..3092678 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K3072_RS15015
• Protein ID: WP_001546171.1
• Coordinates: 3092767..3093135 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K3072_RS14975 (3087892)	3087892..3088446	-	555	WP_001001909.1	molecular chaperone YcdY	-
K3072_RS14980 (3088470)	3088470..3089207	-	738	WP_000283664.1	zinc-binding phosphatase	-
K3072_RS14985 (3089262)	3089262..3090200	-	939	WP_000351287.1	glyoxylate/hydroxypyruvate reductase GhrA	-
K3072_RS14995 (3090671)	3090671..3091514	-	844	Protein_2956	DUF4942 domain-containing protein	-
K3072_RS15000 (3091599)	3091599..3091796	-	198	WP_000772027.1	DUF957 domain-containing protein	-
K3072_RS15005 (3091816)	3091816..3092304	-	489	WP_001546173.1	DUF5983 family protein	-
K3072_RS15010 (3092301)	3092301..3092678	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
K3072_RS15015 (3092767)	3092767..3093135	-	369	WP_001546171.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K3072_RS15020 (3093185)	3093185..3093829	-	645	WP_000094915.1	hypothetical protein	-
K3072_RS15025 (3093848)	3093848..3094069	-	222	WP_000692329.1	DUF987 domain-containing protein	-
K3072_RS15030 (3094132)	3094132..3094608	-	477	WP_001537421.1	RadC family protein	-
K3072_RS15035 (3094624)	3094624..3095097	-	474	WP_000855076.1	antirestriction protein	-
K3072_RS15040 (3095360)	3095360..3096181	-	822	WP_001234710.1	DUF932 domain-containing protein	-
K3072_RS25300 (3096360)	3096360..3096449	-	90	WP_230607454.1	DUF905 family protein	-
K3072_RS15050 (3096592)	3096592..3097047	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T212570 WP_000854690.1 NZ_CP081010:c3092678-3092301 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T212570 NZ_CP107284:159903-160088 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCGTCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13745.37 Da
• Isoelectric Point: 4.6220

>AT212570 WP_001546171.1 NZ_CP081010:c3093135-3092767 [Escherichia coli]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGQFSDADAYHLDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT212570 NZ_CP107284:160105-160515 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACTACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: S1PLF5