Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 2997941..2998412 Replicon chromosome
Accession NZ_CP081010
Organism Escherichia coli strain 25POL

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag K3072_RS14495 Protein ID WP_001303511.1
Coordinates 2998134..2998412 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag K3072_RS14490 Protein ID WP_001302048.1
Coordinates 2997941..2998132 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K3072_RS14445 (2993190) 2993190..2993408 - 219 WP_000209148.1 DUF4014 family protein -
K3072_RS14450 (2993441) 2993441..2993653 - 213 WP_000072553.1 hypothetical protein -
K3072_RS14455 (2993759) 2993759..2994181 - 423 Protein_2849 DUF977 family protein -
K3072_RS14460 (2994197) 2994197..2994967 - 771 WP_000450998.1 DUF1627 domain-containing protein -
K3072_RS14465 (2994989) 2994989..2995735 - 747 WP_000788950.1 ATP-binding protein -
K3072_RS14470 (2995742) 2995742..2996704 - 963 WP_000095673.1 helix-turn-helix domain-containing protein -
K3072_RS14475 (2996727) 2996727..2997152 - 426 WP_000693943.1 toxin YdaT family protein -
K3072_RS14480 (2997149) 2997149..2997451 - 303 WP_001556930.1 transcriptional regulator -
K3072_RS14485 (2997549) 2997549..2997920 + 372 WP_001169687.1 hypothetical protein -
K3072_RS14490 (2997941) 2997941..2998132 + 192 WP_001302048.1 hypothetical protein Antitoxin
K3072_RS14495 (2998134) 2998134..2998412 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
K3072_RS14500 (2998704) 2998704..2998856 + 153 WP_000380313.1 DUF1391 family protein -
K3072_RS14505 (2998868) 2998868..2999206 + 339 WP_000394541.1 hypothetical protein -
K3072_RS14510 (2999195) 2999195..2999389 - 195 WP_001295058.1 hypothetical protein -
K3072_RS14515 (2999965) 2999965..3000153 + 189 WP_000449175.1 cell division inhibition protein DicB -
K3072_RS14520 (3000150) 3000150..3000338 + 189 WP_000199475.1 DUF1482 family protein -
K3072_RS14525 (3000431) 3000431..3002869 + 2439 WP_000102132.1 exonuclease -
K3072_RS14530 (3002931) 3002931..3003200 + 270 WP_000003742.1 excisionase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2980544..3007595 27051


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T212569 WP_001303511.1 NZ_CP081010:2998134-2998412 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T212569 NZ_CP107282:974173-975477 [Vibrio natriegens]
ATGAATGGCGTAAAGGTTGGGGTACTTGAAAAGCGCACAAACGGCGCACATGTTTTCACTTACGATTCGGGTTGGGTTTT
AGCACCAAATGGTCGTCCTATCTCGCTTTCTATGCCTCTCAGGAAGGATGCGTATACTGGCAGTGAGGTTATTAACTTCT
TTGATAACTTACTGCCAGATAATCAGCAGATACGAGATAGAATAGCTGCTCGCTATAAAGCAGATACTAAACAGCCATTC
GACCTATTAGCTAAGGTTGGTCGAGATGCTGTAGGTGCTATCACGCTAGTTCCAGAGGGCTTTGATGTAGATGATCATCG
AACCATAACCGCAAAGGCGTTATCCGAAAAGGAATTGGTTAGAATCCTAAAGGGATATATGGAGGACGCTCCTTTGGGGA
TGCTGAAAGAGGAAGATGATTTTAGGATCTCGATAGCGGGAGCGCAGGAAAAGACAGCCTTACTGTTCTACGATGGAAGA
TGGATGCTACCACAGGGAATGACTCCAACTACGCATATTATCAAGCTGCCAATAGGTGAAATTGTTTCTCAGAACAATGT
TATTGATTTGTCTGCTAGTGTAGATAATGAGTTGATTTGTATGAGGCTAGCAAAGGCGTTCGGCATTCAAACGGCAAACT
GTGACATTATTCGTGCTGGTGATGTTCGTGCCTTGTCTGTTGAGCGATTTGACCGAAAATTATCTTCTGATAACAGCTAT
TACTTGCGCTTGCCCCATGAGGATTTTTGCCAGGCAAAAGGGATTCCCTCAGCTAGAAAATATGAAATTGACGGTGGTAT
CGGCATAGAAGAATCTATTGATATTTTGCGATACTCATACGAAATCAACGATACCGTTACTTTTGCGCGTTCTCAGATAT
TGTTTTGGCTACTAGCTGCCATTGACGGGCATGGAAAGAATTTCTCTGTAAGTATAGCCGCATCTGGTCGATATCAGCTT
GCACCACTGTATGACATTATTTCGGCACATCCACTGACAGGTGGGCGTGGTTTGAGTGAGCGTAAGATCAAGATGGCAAT
GAGCTTACGTGGCACTAGTGGTCGAAAATGGAAGTGGCACCAACTTCAACCGCGCCATTTCGAAGATACCTTTCACACTA
TGAACGTCCCGAACATAAGGCGAGAAAAGGTTCGCTTATTGCTCGATGAGTTTGAATCGACGGCAATGGAGGTGATTGAT
TCTGTCGGAAACTCGCTACCAACTGATATCAATGAAAGCGTAAGAGATAAGATATTTAACGGTGTCTTAAAGACCGTCAA
GAAAATTCAATTGACAAAAAAATAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT212569 WP_001302048.1 NZ_CP081010:2997941-2998132 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT212569 NZ_CP107282:973916-974155 [Vibrio natriegens]
ATGCTAGTCAACACACCGACAGCCCTCGGCAATGCGTTACGCGAAGCCCGAAAGGAAAGCGGCCTAAAGCAAACAGATCT
TGGTCTGCGACAAGCAACAGTATCTAATTTTGAAAGCAACCCTGAAAAATCCACGATAGAAACATTGTTCAAGCTCCTGT
CTATCAATGGACTAGAAATGCATATTGTGCCGAAAGGCAAGCACATTACTGAAACTAAAGGGGCTGTTGACGAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References